Journal of South China University of Technology(Natural Science Edition) ›› 2019, Vol. 47 ›› Issue (8): 64-70.doi: 10.12141/j.issn.1000-565X.180097

• Food Science & Technology • Previous Articles     Next Articles

Reversal Effect of Sertraline on Multidrug Resistance of Staphylococcus aureus

MENG Hecheng1 XIE Shenghai1 LI Lili2 CHEN Miaorui3   

  1. 1． School of Food Science and Engineering，South China University of Technology，Guangzhou 510640，Guangdong，China; 2． Food Safety and Nutrition Research Institute，Jinan University，Guangzhou 510632，Guangdong，China; 3． Xiamen Hongyi Testing Co． ，Ltd． ，Xiamen 361100，Fujian，China
  • Received:2019-03-18 Revised:2019-04-24 Online:2019-08-25 Published:2019-08-01
  • Contact: 李丽丽(1985-)，女，博士，讲师，主要从事快速检测与细菌耐药性研究． E-mail:lilili2017@jnu.edu.cn
  • About author:孟赫诚(1984-)，男，博士，讲师，主要从事快速检测与细菌耐药性研究． E-mail:femenghc@ scut． edu． cn
  • Supported by:
    Supported by the Agricultural Science and Technology Innovation and Promotion Project of Guangdong Province (2018LM2172) and the Science and Technology Planning Project of Guangdong Province(2017B020207004)

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Abstract: Multidrug-resistant Staphylococcus aureus (S. aureus) were taken as object to investigate the reversal effect of sertraline on multi-drug resistance of S. aureus and its reversal mechanism through the determination of minimum inhibitory concentration，growth rate，time-kill curves under the combination of non-antibiotics sertraline and different antibiotics，as well as transcriptional level of the drug resistance gene mecA and the efflux pump gene norA by real-time PCR． The results indicate that when the sertraline concentration is higher than 1 μg/mL，the growths of S. aureus are inhibited; when sertraline (1/2 MIC) is combined with tetracycline，it has a bactericidal effect on S. aureus． Sertraline combined with tetracycline，ciprofloxacin，and clindamycin，it has synergistic or additive inhibitory effect on S. aureus，which could increase the sensitivity of three antibiotics by more than 75%． Low concentration of sertraline could significantly reduce the expression of the efflux pump gene norA and mecA gene in SCCmec gene cassettes of S. aureus． The results show that sertraline can regulate the multi-drug resistance of S. aureus by inhibiting the expression of resistance genes such as norA and mecA，and reverse the resistance of S. aureus to various antibiotics when it is combined with antibiotics．

Key words: multi-drug resistance, sertraline, efflux pump, SCCmec gene cassette

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