重组降血压肽多肽序列的设计及表达系统构建

华南理工大学学报（自然科学版） ›› 2005, Vol. 33 ›› Issue (10): 90-94.

重组降血压肽多肽序列的设计及表达系统构建

刘冬¹ 李世敏² 张丽君² 唐语谦¹ 梁世中¹

1．华南理工大学生物科学与工程学院，广东广州 510640；2．深圳职业技术学院应用化学与生物技术学院，广东深圳 518055

收稿日期:2004-12-08 出版日期:2005-10-25 发布日期:2005-10-25
通信作者: 刘冬(1968-)，男，博士生，深圳职业技术学院副教授，主要从事生物技术方面的研究 E-mail:liudongsz@oa.szpt.net
作者简介:刘冬(1968-)，男，博士生，深圳职业技术学院副教授，主要从事生物技术方面的研究
基金资助:
广东省社会发展攻关资助项目(20032979)；深圳市科技计划资助项目(03KJ6021)

Design of Recombined Angiotensin-Converting Enzyme Inhibitor Peptide Sequence and Construction of Expression System

Liu Dong¹ Li Shi-min² Zhang Li-jun² Tang Yu-qian¹ Liang Shi-zhong¹

1.College of Biological Science and Engineering,South China Univ.of Tech.,Guangzhou 510640,Guangdong,China;2.School of Applied Chemistry and Biological Technology,Shenzhen Polytechnic,Shenzhen 518055,Guangdong,China

Received:2004-12-08 Online:2005-10-25 Published:2005-10-25
Contact: 刘冬(1968-)，男，博士生，深圳职业技术学院副教授，主要从事生物技术方面的研究 E-mail:liudongsz@oa.szpt.net
About author:刘冬(1968-)，男，博士生，深圳职业技术学院副教授，主要从事生物技术方面的研究
Supported by:
广东省社会发展攻关资助项目(20032979)；深圳市科技计划资助项目(03KJ6021)

摘要/Abstract

摘要： 通过分析降血压肽结构与功能的关系，设计并合成5条降血压肽序列．通过活性测定以及体外消化试验，确定P₃ (六肽)为理想降血压肽．为实现该小肽在大肠杆菌中的表达，经特定酶切位点将其串联为6拷贝的融合多肽，将相应的基因序列克隆至表达载体pGEX-4T-2并转化至宿主茵Escherichia coli BL21中．双酶切、PCR以及测序结果均表明成功构建了重组质粒pGEX-4T-2-ACEIP．

关键词: 降血压肽, 序列设计, 基因重组

Abstract:

Five angiotensin-converting enzyme inhibitor peptide(ACEIP)sequences were first designed and synthesized according to the relationship between the function and the structure of ACEIP.Next,the optimal peptide,namely,P₃(six-amino-acid sequence)was determined by the bioactivity measurement and the digesting test in vitro.It was then jointed at the specific enzyme cleavage site to construct a six-copy fusion peptide.The corresponding coding gene was cloned into pGEX4T-2.The fusion expression vector of pGEX4T-2-ACEIP was finally transformed into Escherichia coli BL21.The results of the double enzyme-cleavage,PCR an d the sequence analysis all indicate that the recombinant plasmid pGEX-T-2-ACEIP is successfully constructed.

Key words: angiotensin-converting enzyme inhibitor peptide, sequence design, gene recombination

刘冬李世敏张丽君唐语谦梁世中. 重组降血压肽多肽序列的设计及表达系统构建[J]. 华南理工大学学报（自然科学版）, 2005, 33(10): 90-94.

Liu Dong Li Shi-min Zhang Li-jun Tang Yu-qian Liang Shi-zhong. Design of Recombined Angiotensin-Converting Enzyme Inhibitor Peptide Sequence and Construction of Expression System[J]. Journal of South China University of Technology (Natural Science Edition), 2005, 33(10): 90-94.