阿维拉霉素高产菌株的推理选育

华南理工大学学报（自然科学版） ›› 2007, Vol. 35 ›› Issue (4): 100-104,116.

阿维拉霉素高产菌株的推理选育

朱传合¹贺亚男²杜连祥²路福平²

1.山东农业大学食品科学与工程学院，山东泰安 271018; 2. 天津科技大学生物工程学院，天津 30022

收稿日期:2006-03-24 出版日期:2007-04-25 发布日期:2007-04-25
通信作者: 朱传合(1978-)，男，博士，主要从事工业微生物育种和微生物制药方面的研究. E-mail:chuanhezhu@yahoo. com. cn
作者简介:朱传合(1978-)，男，博士，主要从事工业微生物育种和微生物制药方面的研究.
基金资助:
天津市科技攻关重点专项(05YFGZGX04400)

Rational Screening of High -Yield A vilamycin-Producing Strain

Zhu Chuan-he¹ He Ya-nan² Du Lian-xiang² Lu Fu-pin²

1. College of Food Science and Engineering , Shandong Agricultural Univ. , Taian 271018 , Shandong ,China;2. College of Biotechnology , Tianjin Univ. of Science and Tech. , Tianjin 300222 , China

Received:2006-03-24 Online:2007-04-25 Published:2007-04-25
Contact: 朱传合(1978-)，男，博士，主要从事工业微生物育种和微生物制药方面的研究. E-mail:chuanhezhu@yahoo. com. cn
About author:朱传合(1978-)，男，博士，主要从事工业微生物育种和微生物制药方面的研究.
Supported by:
天津市科技攻关重点专项(05YFGZGX04400)

摘要/Abstract

摘要： 获得阿维拉霉素高产菌株，在研究菌株Streptomyces viridocbromogenes 96 (SV-96) 葡萄糖及氨盐代谢的基础上，采用"NTG 随机诱变+氨基乙酸和2-脱氧-D 葡萄糖联合抗性"的试验方案进行阿维拉霉素高产茵株的推理选育，获得了氨基乙酸和2-脱氧-D葡萄糖联合抗性高产突变株SV-GT-596. 该菌株遗传性能良好，阿维拉霉素产量高达56.4mg/L ，较出发菌株提高了162.3% .实验表明，氨基乙酸和2-脱氧-D- 葡萄糖抗性推理选育可克服随机筛选的盲目性，提高筛选工作效率.

关键词: 阿维拉霉素, 推理选育, 氨基乙酸2-脱氧-D-葡萄糖, 绿色产色链霉菌

Abstract:

In order to screen high-yield avilamycin-producing strain , a scheme of "random NTG mutation + glycine and 2-deoxy-D-glucose resistant rational screening" was proposed after investigating the metabolism of glucose and ammonium by Streptomyces viridocbromogenes 96 (SV -96). A glycine and 2-deoxy-D-glucose resistant mutant (SV-GT-596) with high yield was then obtained. It is found that SV -GT -596 strain is of good transmissibility， and that ,by using the strain , the yield of avilamycin reaches 56. 4 mg/L, which is 62. 3 % more than the initial yield by flake fermentation. Experimental results also indicate that the rational screening overcomes the blindness of random screening and improves the screening efficiency.

Key words: avilamycin, rational screening, glycine, 2-deoxy-D-glucose, Streptomyces viridocbromogenes

朱传合贺亚男杜连祥路福平. 阿维拉霉素高产菌株的推理选育[J]. 华南理工大学学报（自然科学版）, 2007, 35(4): 100-104,116.

Zhu Chuan-he He Ya-nan Du Lian-xiang Lu Fu-pin. Rational Screening of High -Yield A vilamycin-Producing Strain[J]. Journal of South China University of Technology (Natural Science Edition), 2007, 35(4): 100-104,116.