关键词: 三元配合物, 邻菲咯啉, 氨基酸, 铜, DNA, 插入作用

Abstract:

The interactions between DNA and three complexes,namely,[Cu(phen)(L-Gly)(H₂O)]•CLO₄•2.5H₂O(phen=phenanthroline,L-Gly=L-Glycine),[Cu(phen)(L-Ile)(H₂O)](CLO₄)(L-Ile=Isoleucine)and[Cu(phen)(L-Met)(H₂O)](CLO₄)(L-Met=L-Methionine),were investigated by means of the nu0rescence
spectroscopy,the electronic absorption spectroscopy,the viscosity measurement and the agarose gel electrophore.sis.It is revealed by the fluorescence spectroscopy.the electronic ab sorption spectroscopy and the viscosity men.surement that the mode of the interaction between the complexes and DNA is partial intercalation.Moreover,the results of agarose gel electrophoresis indicate that all the complexes exhibit obvious pBR322 DNA cleavage activitv.Furthermore,a moderate size of the auxiliary ligand should be taken into consideration because a larger or snlaller steric hindrance of the auxiliary ligand would afect the interaction between the three designed complexes and DNA.

Key words: ternary complex, 1,10-phenanthroline, amino acid, copper, DNA, intercalati0n