关键词: PCR - 单链构象多态性, 微生物多样性, 筛选, SJ培养基, 高效脱酚菌

Abstract:

In order to reveal the differences in the microbial diversity of the bacilli stock cultured in different sub-strates aswell as culture high2efficiency phenol2degradation bacteria, the V3 section during the 16S rRNA was used as the target to investigate and compare the biodiversities of bacteria isolates respectively cultured in three different isolation substrates, namely the self-prepared SJ substrate, the LB substrate and the YEB substrate. The results show that SJ substrate ismore suitable for the isolation of bacteria with higher microbial diversity than LB and YEB substrates, and that the three strains of high2efficiency phenol-degradation bacteria screened by SJ substrate are all of a total-phenol removal rate ofmore than 99%. Moreover, according to the phylogenic tree established bymeans of physiological and biochemical app raisals and 16S rDNA sequencing, the three strains are respectively Aero-monas, Bacillus and Acinetobacter.

Key words: PCR-SSCP, microbial diversity, screening, SJ substrate, high-efficiency phenol-degradation bacterium