关键词: 脂质体, 磷脂, 偶联, 直链醛

Abstract:

In order to easily make proteins,such as antibodies,cross-link on the surface of liposome and control the covered area on the surface,a simple and controllable preparation of immunized liposome was,presented.In the course of preparation,linear-chain aldehyde with certain proportion was added into the mixture of phosphatidylch-line and cholesterol,and a reverse-phase evaporation technique was adopted to produce liposomes.The results indi-cate that the hydrophilic aldehyde groups arranged in the bilayer lipid molecules expose to the aqueous phase and react with the amino groups in the antibody molecules,forming Schif base,that the covering area can be changed by changing the amount of aldehyde,and that the activity of the liposome is greatly improved.

Key words: liposome, phosphatide, coupling, linear-chain aldehyde