关键词: 菌落 PCR, 常规 PCR, tdh 基因 , tlh 基因

Abstract: To establish a quick, sensitive and economical PCRmethod for directly detecting tlh and adh genes-carrying Vibrio parahaemolyticus in environment and seafood, a colony PCR method was developed and its resultswere compared with those of the conventional PCRmethod, thus establishing a colony PCRmethod.By using the Vibrioparahaemolytic-us strains as positive controls, several marine bacteria strains preserved in the lab were amplified through the colony PCR method and the conventional PCR method , by which the reliability of colony PCR method for the corresponding gene de-tection was proved.The comparison shows that the results obtained by colony PCR method are in great agreement with those obtained by the conventional PCRmethod, and that the tlh and tdh genes in positive controls can be amplified by these two PCR methods.

Key words: colony PCR, conventional PCR, tdh gene, tlh gene