甜菜果胶中性糖的水解方法及定量分析

doi:10.3969/j.issn.1000-565X.2017.09.008

华南理工大学学报（自然科学版） ›› 2017, Vol. 45 ›› Issue (9): 53-59.doi: 10.3969/j.issn.1000-565X.2017.09.008

甜菜果胶中性糖的水解方法及定量分析

于淑娟^1,2 王智明¹郭晓明^1,2李太鸿¹ 艾超¹ 郭筱兵¹

1．华南理工大学食品科学与工程学院，广东广州 510640; 2．华南理工大学广东省天然产物绿色加工与产品安全重点实验室，广东广州 510640

收稿日期:2017-02-20 修回日期:2017-06-29 出版日期:2017-09-25 发布日期:2017-08-30
通信作者: 于淑娟( 1955-) ，女，教授，博士生导师，主要从事制糖工程、碳水化合物分子修饰研究． E-mail:lfshjy@scut.edu.cn
作者简介:于淑娟( 1955-) ，女，教授，博士生导师，主要从事制糖工程、碳水化合物分子修饰研究．
基金资助:
国家自然科学基金资助项目( 31601423 ) ; 广东省科技计划项目( 2013B051000015 ) ; 兵团科技攻关项目( 2016AB009) ; 中国博士后科学基金资助项目( 2016M590789)

A Hydrolyzing Method of Neutral Sugar in Sugar Beet Pectin and Their Quantitative Analysis

YU Shu-juan^1,2 WANG Zhi-ming¹GUO Xiao-ming^1,2 LI Tai-hong¹ AI Chao¹ GUO Xiao-bing¹

1.School of Food Science and Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China; 2.Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety,South China University of Technology,Guangzhou 510640,Guangdong,China

Received:2017-02-20 Revised:2017-06-29 Online:2017-09-25 Published:2017-08-30
Contact: 于淑娟( 1955-) ，女，教授，博士生导师，主要从事制糖工程、碳水化合物分子修饰研究． E-mail:lfshjy@scut.edu.cn
About author:于淑娟( 1955-) ，女，教授，博士生导师，主要从事制糖工程、碳水化合物分子修饰研究．
Supported by:
Supported by the National Natural Science Foundation of China ( 31601423) ， the Science and Technology Planning Project of Guangdong Province ( 2013B051000015) ， the Achievements Transformation Fund Project ( 2016AB009) and China Postdoctoral Science Foundation( 2016M590789)

摘要/Abstract

摘要： 为测定甜菜果胶中性糖的种类及含量，建立了针对甜菜果胶中性糖的两步水解法．该法采用果胶酶、三氟乙酸( TFA) 依次水解甜菜果胶的中性糖．首先，甜菜果胶被果胶酶水解为相对分子质量较小的组分; 其次，在TFA 作用下，中性单糖从甜菜果胶分子上逐步释放到水解液中．在受试的3 种商业酶制剂中，果胶酶Ｒapidase C80 因其酶解效果好、内源性单糖少等优点被选为目的果胶酶．研究结果表明，C80 的最适酶解条件为50 ℃、pH5、15 h．在C80 酶解的基础上，进一步研究TFA 浓度、酸解温度、酸解时间对中性糖水解效果的影响．综合评价中性糖水解率与降解率的关系后，确定适宜的TFA 浓度、温度、时间分别为1mol /L、110℃、4 h．高效阴离子交换色谱( HPAEC) 分析结果表明，两步水解法对鼠李糖的水解效果显著，且该法稳定性好、可重复性高，能够满足甜菜果胶中性糖定量分析的需要．

关键词: 甜菜果胶, 中性糖, 两步水解, 离子色谱

Abstract: In order to determine the variety and contents of the neutral sugar in sugar beet pectin ( SBP) ,a twostep hydrolysis method was proposed for the SBP.In the method,the neutral sugar in the SBP were sequentially hydrolyzed by using pectinase and trifluoroacetic acid ( TFA) .First,the SBP was degraded into the fractions of smaller relative molecular mass by pectinase.Second,the neutral sugar attached to the SBP were liberated into solution by TFA.Among three commercial pectinases,Rapidase C80 was selected as the objective pectinase because of its lower endogenous monosaccharide content and better hydrolyzing activity.It is found that the optimal hydrolysis parameters of C80 are 50℃,pH 5 and 15 h.Moreover,the effects of the TFA concentration,the temperature and the hydrolysis duration on the hydrolysis of the neutral sugar were further investigated.By compromising the hydrolysis rate and the degradation rate of the neutral sugar,suitable TFA concentration,temperature and hydrolysis duration were respectively determined to be 1mol /L,110℃ and 4 h.The HPAEC analysis results show that the proposed method has a good effect in achieving rhamnose through hydrolysis,and it exhibits a good stability and a high repeatability,which means that this method can be used to quantitatively analyze the neutral sugar in the SBP.

Key words: sugar beet pectin, neutral sugar, two-step hydrolysis, ion chromatography

中图分类号:

TS201

于淑娟王智明郭晓明李太鸿艾超郭筱兵. 甜菜果胶中性糖的水解方法及定量分析[J]. 华南理工大学学报（自然科学版）, 2017, 45(9): 53-59.

YU Shu-juan WANG Zhi-ming GUO Xiao-ming LI Tai-hong AI Chao GUO Xiao-bing. A Hydrolyzing Method of Neutral Sugar in Sugar Beet Pectin and Their Quantitative Analysis[J]. Journal of South China University of Technology (Natural Science Edition), 2017, 45(9): 53-59.

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[2]	于淑娟刘定波徐献兵吴鑫兰. 离子色谱－质谱联用法测定 4-甲基咪唑的含量[J]. 华南理工大学学报（自然科学版）, 2015, 43(2): 8-12.

甜菜果胶中性糖的水解方法及定量分析

A Hydrolyzing Method of Neutral Sugar in Sugar Beet Pectin and Their Quantitative Analysis

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