复合富血小板血浆和贫血小板血浆冻干粉的磷酸钙骨水泥的细胞响应

doi:10.3969/j.issn.1000-565X.2014.10.001

华南理工大学学报（自然科学版） ›› 2014, Vol. 42 ›› Issue (10): 1-6.doi: 10.3969/j.issn.1000-565X.2014.10.001

• 材料科学与技术 • 下一篇

复合富血小板血浆和贫血小板血浆冻干粉的磷酸钙骨水泥的细胞响应

叶建东^1,2 吴华娥^1,2 何福坡³ 张婧^1,2

1.华南理工大学材料科学与工程学院，广东广州 510640； 2.华南理工大学国家人体组织功能 重建工程技术研究中心，广东广州 510006； 3.广州医科大学基础学院，广东广州 510182

收稿日期:2014-04-01 修回日期:2014-04-30 出版日期:2014-10-25 发布日期:2014-10-01
通信作者: 叶建东（1959-），男，教授，博士生导师，主要从事生物医用材料研究. E-mail:jdye@scut.edu.cn
作者简介:叶建东（1959-），男，教授，博士生导师，主要从事生物医用材料研究.
基金资助:
国家自然科学基金资助项目（51172074）

Cell Response to Calcium Phosphate Cement Incorporating Lyophilized Powders of PRP and PPP

Ye Jian-dong^1,2 Wu Hua-e^1,2 He Fu-po³ Zhang Jing^1,2

1. School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China; 2. National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, Guangdong, China; 3. School of Basic Science, Guangzhou Medical University, Guangzhou 510182, Guangdong, China

Received:2014-04-01 Revised:2014-04-30 Online:2014-10-25 Published:2014-10-01
Contact: 叶建东（1959-），男，教授，博士生导师，主要从事生物医用材料研究. E-mail:jdye@scut.edu.cn
About author:叶建东（1959-），男，教授，博士生导师，主要从事生物医用材料研究.
Supported by:
国家自然科学基金资助项目（51172074）

摘要/Abstract

摘要： 用冷冻干燥制得的富血小板血浆（PRP）和贫血小板血浆（PPP）干粉对磷酸钙骨水泥（CPC）进行改性，采用X射线衍射、扫描电镜和细胞实验等手段研究了PRP和PPP对CPC水化产物物相、断面显微形貌和细胞响应特性的影响.结果表明：复合PRP或PPP并不影响CPC的水化产物，最终水化产物均是弱结晶的羟基磷灰石；复合一定量的PRP或PPP对CPC表面人骨髓间充质干细胞的粘附、增殖和分化具有明显的促进作用，在细胞培养的后期，复合较少的PRP对细胞增殖和分化具有更明显的促进作用.这表明PRP和PPP均有望用于提高CPC的细胞响应能力和骨诱导性.

关键词: 富血小板血浆, 贫血小板血浆, 磷酸钙骨水泥, 细胞响应, 骨诱导性

Abstract:

Calcium phosphate cement (CPC) was modified by incorporating the lyophilized powders of plateletrich plasma (PRP) and plateletpoor plasma (PPP), and the effects of PRP or PPP on the hydration product phase, section morphology and cell response of CPC were characterized by means of XRD, SEM and cell experiments. The results show that (1) incorporating PRP or PPP does not affect the hydration products of CPC, and the final hydration product is nothing but poorly crystalline hydroxyapatite; (2) incorporating an appropriate amount of lyophilized powders of PRP or PPP into CPC greatly promotes the adhesion, proliferation and differentiation of human mesenchymal stem cells (hMSCs) on CPC; and (3) in the late stage of cell culture, incorporating a smaller amount of PRP helps to improve cell proliferation and differentiation. Therefore, it is expected that PRP and PPP can be used to improve the cell response and osteoinductivity of CPC.

Key words: platelet-rich plasma, platelet-poor plasma, calcium phosphate cement, cell response, osteoinductivity

叶建东吴华娥何福坡张婧. 复合富血小板血浆和贫血小板血浆冻干粉的磷酸钙骨水泥的细胞响应[J]. 华南理工大学学报（自然科学版）, 2014, 42(10): 1-6.

Ye Jian-dong Wu Hua-e He Fu-po Zhang Jing. Cell Response to Calcium Phosphate Cement Incorporating Lyophilized Powders of PRP and PPP[J]. Journal of South China University of Technology (Natural Science Edition), 2014, 42(10): 1-6.

复合富血小板血浆和贫血小板血浆冻干粉的磷酸钙骨水泥的细胞响应

Cell Response to Calcium Phosphate Cement Incorporating Lyophilized Powders of PRP and PPP

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价