舍曲林对副溶血弧菌的抑制作用

doi:10.12141/j.issn.1000-565X.230183

华南理工大学学报(自然科学版) ›› 2023, Vol. 51 ›› Issue (12): 131-139.doi: 10.12141/j.issn.1000-565X.230183

舍曲林对副溶血弧菌的抑制作用

孟赫诚¹ 何长衡¹ 蔡萍瑶¹ 李丽丽²

^1.华南理工大学食品科学与工程学院，广东广州 510640
^2.暨南大学食品安全与营养研究院，广东广州 510632

收稿日期:2023-04-04 出版日期:2023-12-25 发布日期:2023-07-14
通信作者: 李丽丽（1985-），讲师，主要从事微生物耐药研究。 E-mail:lilili2017@jnu.edu.cn
作者简介:孟赫诚（1984-），男，副教授，主要从事食品安全研究。E-mail： femenghc@scut.edu.cn
基金资助:
国家自然科学基金资助项目(32001796);广州市基础与应用基础研究项目(202002030145)

Inhibitory Effect of Sertraline on Vibrio Parahaemolyticus

MENG Hecheng¹ HE Changheng¹ CAI Pingyao¹ LI Lili²

^1.School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China
^2.Institute of Food Safety and Nutrition, Jinan University, Guangzhou 510632, Guangdong, China

Received:2023-04-04 Online:2023-12-25 Published:2023-07-14
Contact: 李丽丽（1985-），讲师，主要从事微生物耐药研究。 E-mail:lilili2017@jnu.edu.cn
About author:孟赫诚（1984-），男，副教授，主要从事食品安全研究。E-mail： femenghc@scut.edu.cn
Supported by:
the National Natural Science Foundation of China(32001796)

摘要/Abstract

摘要：

近年来关于副溶血弧菌环境和海产品分离株出现抗生素耐药性的报道日益增多，亟需寻找替代传统抗菌剂抗菌的策略以控制副溶血弧菌的污染和感染。本研究通过最小抑菌浓度（MIC）和最低杀菌浓度（MBC）的测定、生长曲线的绘制，以及运动性实验和结晶紫染色实验，并利用透射电镜观察副溶血弧菌形态的变化，来评估以舍曲林为代表的5-羟色胺再摄取抑制剂（SSRIs）类药物对副溶血弧菌的抑制活性及抗生物被膜形成能力。同时，通过检测与分析舍曲林对副溶血弧菌毒力基因转录的影响，探究舍曲林对副溶血弧菌的减毒作用。结果显示，舍曲林对副溶血弧菌的MIC为32 μg/mL，MBC为64 μg/mL，能够损伤其细胞膜和细胞壁，MIC下导致其干瘪皱缩，MBC下引起其涨裂、胞内物质外泄；亚抑制浓度的舍曲林能显著抑制副溶血弧菌的泳动和群集运动，抑制率分别为88.6%和71.5%；舍曲林呈浓度依赖性地抑制副溶血弧菌的生物被膜形成，当舍曲林质量浓度分别为8、16、32和64 μg/mL时，对副溶血弧菌生物被膜形成的抑制率分别为66.9%、79.5%、88.3%和89.3%；亚抑制浓度的舍曲林能显著抑制副溶血弧菌的毒力基因fliA、ompW和aphA的表达，抑制率分别为71.9%、88.7%和77.3%。实验结果表明，舍曲林对副溶血弧菌具有良好的抗菌活性与抗生物被膜形成能力，在不影响副溶血弧菌生长的浓度下，可降低其毒力基因的转录水平。

关键词: 舍曲林, 副溶血弧菌, 运动性, 毒力基因, 群体感应

Abstract:

Recently, there have been increasing reports on the emergence of antibiotic resistance in environmental and seafood isolates of Vibrio parahaemolyticus. Therefore, it is urgent to find strategies to replace traditional antimicrobial agents for antibacterial control of Vibrio parahaemolyticus contamination and infection. In this study, the antibacterial activity and anti-biofilm formation ability of sertraline against Vibrio parahaemolyticus were evaluated through minimum inhibitory concentration (MIC) test, minimum bactericidal concentration (MBC) test, growth curve test, motivity test, and crystal violet staining test, and transmission electron microscope observation of the morphological changes of Vibrio parahaemolyticus. Meanwhile, the attenuating effect of sertraline on Vibrio parahaemolyticus was explored by detecting and analyzing the influence of sertraline on the transcription of virulence genes of Vibrio parahaemolyticus. The results indicate that the MIC of sertraline on Vibrio parahaemolyticus is 32 μg/mL and the MBC is 64 μg/mL, which are able to damage the cell membrane and cell wall of Vibrio parahaemolyticus. Furthermore, sertraline can lead to shrinkage and shriveling at MIC concentration, and rupture and leakage of intracellular substances at MBC concentration. Sertraline can significantly inhibit the swimming and swarm motion of Vibrio parahaemolyticus with the inhibition rates of 88.6% and 71.5%, respectively, at the subinhibitory concentration. The ability of sertraline to inhibit biofilm formation of Vibrio parahaemolyticus shows a linear correlation of sertraline’s concentration, and the inhibition rates of 8, 16, 32 and 64 μg/mL sertraline on the biofilm formation of Vibrio parahaemolyticus ATCC17802 are 66.9%, 79.5%, 88.3% and 89.3%, respectively. Sertraline can significantly inhibit the expression of virulence genes fliA, ompW and aphA of Vibrioparahaemolyticus with inhibition rates of 71.9%, 88.7% and 77.3% at subinhibitory concentration, respectively. The results show that sertraline has a good antibacterial activity and anti-biofilm formation ability against Vibrio parahaemolyticus, and it can reduce the transcription level of virulence genes under the allowable concentration of plasma without affecting its growth.

Key words: sertraline, Vibrio parahaemolyticus, motility, virulence gene, quorum sensing

中图分类号:

R446.5

孟赫诚, 何长衡, 蔡萍瑶, 等. 舍曲林对副溶血弧菌的抑制作用[J]. 华南理工大学学报(自然科学版), 2023, 51(12): 131-139.

MENG Hecheng, HE Changheng, CAI Pingyao, et al. Inhibitory Effect of Sertraline on Vibrio Parahaemolyticus[J]. Journal of South China University of Technology(Natural Science Edition), 2023, 51(12): 131-139.

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基因	引物序列（5'-3'）	参考文献
fliA-F	TAAGCGTATTGCTCACCACCT	［27］
fliA-R	GCTCGCACCTTTAGAACCAT	［27］
ompW-F	TCGTGTCACCAAGTGTTTTCG	［28］
ompW-R	CGTGGCTGAATGGTGTTGC	［28］
aphA-F	ACACCCAACCGTTCGTGATG	［28］
aphA-R	GTTGAAGGCGTTGCGTAGTAAG	［28］
pvuA-F	CAAACTCACTCAGACTCCA	［29］
pvuA-R	CGAACCGATTCAACACG	［29］

舍曲林对副溶血弧菌的抑制作用

Inhibitory Effect of Sertraline on Vibrio Parahaemolyticus

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菌株名称	MIC/（μg·mL^-1）	MBC/（μg·mL^-1）
ATCC17802	32	64
16vp01	32	64
16vp03	32	64
16vp09	32	64
16vp12	32	64
16vp15	32	64
16vp17	32	64
16vp20	32	64
16vp23	32	64
15vp77	32	64
15vp79	32	64
15vp80	32	64