舍曲林对金黄色葡萄球菌多重耐药性的逆转作用

doi:10.12141/j.issn.1000-565X.180097

华南理工大学学报(自然科学版) ›› 2019, Vol. 47 ›› Issue (8): 64-70.doi: 10.12141/j.issn.1000-565X.180097

舍曲林对金黄色葡萄球菌多重耐药性的逆转作用

孟赫诚¹ 谢胜海¹李丽丽^2† 陈妙瑞³

1．华南理工大学食品科学与工程学院，广东广州 510640; 2．暨南大学食品安全与营养研究院，广东广州 510632; 3．厦门泓益检测有限公司，福建厦门 361100

收稿日期:2019-03-18 修回日期:2019-04-24 出版日期:2019-08-25 发布日期:2019-08-01
通信作者: 李丽丽(1985-)，女，博士，讲师，主要从事快速检测与细菌耐药性研究． E-mail:lilili2017@jnu.edu.cn
作者简介:孟赫诚(1984-)，男，博士，讲师，主要从事快速检测与细菌耐药性研究． E-mail:femenghc@ scut． edu． cn
基金资助:
广东省农业科技创新及推广项目(2018LM2172);广东省科技计划项目(2017B020207004);暨南大学科研培育与创新基金研究项目(21618309)

Reversal Effect of Sertraline on Multidrug Resistance of Staphylococcus aureus

MENG Hecheng¹ XIE Shenghai¹ LI Lili²CHEN Miaorui³

1． School of Food Science and Engineering，South China University of Technology，Guangzhou 510640，Guangdong，China; 2． Food Safety and Nutrition Research Institute，Jinan University，Guangzhou 510632，Guangdong，China; 3． Xiamen Hongyi Testing Co．，Ltd．，Xiamen 361100，Fujian，China

Received:2019-03-18 Revised:2019-04-24 Online:2019-08-25 Published:2019-08-01
Contact: 李丽丽(1985-)，女，博士，讲师，主要从事快速检测与细菌耐药性研究． E-mail:lilili2017@jnu.edu.cn
About author:孟赫诚(1984-)，男，博士，讲师，主要从事快速检测与细菌耐药性研究． E-mail:femenghc@ scut． edu． cn
Supported by:
Supported by the Agricultural Science and Technology Innovation and Promotion Project of Guangdong Province (2018LM2172) and the Science and Technology Planning Project of Guangdong Province(2017B020207004)

摘要/Abstract

摘要： 以多重耐药金黄色葡萄球菌为研究对象，通过测定非抗生素类药物舍曲林与不同抗生素作用的最低抑菌浓度、生长速率、时间－杀菌曲线及实时荧光定量 PCＲ定量检测耐药基因 mecA 和外排泵基因 norA 的转录水平，探讨舍曲林对金黄色葡萄球菌多重耐药性的逆转作用及逆转机制．结果显示:当舍曲林质量浓度高于 1 μg/mL 时，对金黄色葡萄球菌有抑制作用;亚抑菌浓度(1/2 MIC)的舍曲林和四环素联用时对金黄色葡萄球菌具有杀菌作用;当舍曲林和四环素、环丙沙星、克林霉素联用时对金黄色葡萄球菌具有协同或相加抑制作用，可使其对 3 种抗生素的敏感性提高 75% 以上;低质量浓度的舍曲林可显著降低外排泵基因 norA 和 SCCmec 基因盒中 mecA 基因的表达水平．实验结果表明，舍曲林能通过抑制 norA 和 mecA 等耐药基因的表达来调控金黄色葡萄球菌的多重耐药性，通过联合抑菌部分逆转金黄色葡萄球菌对多种抗生素的耐药性．

关键词: 多重耐药性, 舍曲林, 外排泵, SCCmec 基因盒

Abstract: Multidrug-resistant Staphylococcus aureus (S. aureus) were taken as object to investigate the reversal effect of sertraline on multi-drug resistance of S. aureus and its reversal mechanism through the determination of minimum inhibitory concentration，growth rate，time-kill curves under the combination of non-antibiotics sertraline and different antibiotics，as well as transcriptional level of the drug resistance gene mecA and the efflux pump gene norA by real-time PCR． The results indicate that when the sertraline concentration is higher than 1 μg/mL，the growths of S. aureus are inhibited; when sertraline (1/2 MIC) is combined with tetracycline，it has a bactericidal effect on S. aureus． Sertraline combined with tetracycline，ciprofloxacin，and clindamycin，it has synergistic or additive inhibitory effect on S. aureus，which could increase the sensitivity of three antibiotics by more than 75%． Low concentration of sertraline could significantly reduce the expression of the efflux pump gene norA and mecA gene in SCCmec gene cassettes of S. aureus． The results show that sertraline can regulate the multi-drug resistance of S. aureus by inhibiting the expression of resistance genes such as norA and mecA，and reverse the resistance of S. aureus to various antibiotics when it is combined with antibiotics．

Key words: multi-drug resistance, sertraline, efflux pump, SCCmec gene cassette

中图分类号:

孟赫诚谢胜海李丽丽陈妙瑞 . 舍曲林对金黄色葡萄球菌多重耐药性的逆转作用[J]. 华南理工大学学报(自然科学版), 2019, 47(8): 64-70.

MENG Hecheng XIE Shenghai LI Lili CHEN Miaorui. Reversal Effect of Sertraline on Multidrug Resistance of Staphylococcus aureus[J]. Journal of South China University of Technology(Natural Science Edition), 2019, 47(8): 64-70.

舍曲林对金黄色葡萄球菌多重耐药性的逆转作用

Reversal Effect of Sertraline on Multidrug Resistance of Staphylococcus aureus

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价