Proposed in this paper is a sub-pixel algorithm based on phase correlation and image resampling for high-accuracy image registration.In this algorithm,first,a pixel-level coarse location is realized using the conventional phase correlation method.Then,a fine step is performed,using the matrix multiplication discrete Fourier transform（DFT） to calculate the resampling region around the coarse point and to further locate the resampling region at a pixel level based on the phase correlation.Moreover,the accuracy equivalence between the matrix multiplication DFT and the zero-padding resampling is proved in detail.Experimental results show that the proposed algorithm is superior to the conventional cross-correlation-based and phase-correlation-based sub-pixel registration algorithms in terms of accuracy,efficiency and noise resistance.