This paper investigates the self-organization conditions and process of networked manufacturing information system （NMIS） based on the self-organization theory and methods. In order to analyze the relationship between macroscopic relative stability and unit action and to reveal the self-organization essentials of NMIS, a servo constraint function is proposed and is then used to illustrate the servo function of macroscopic order parameters for the units＇ self-interaction and interaction. Moreover, by taking the information system of a mould production line in a manufacturing enterprise as the platform, a comparison between the simulation and the operation results is performed, by which the effectiveness of the proposed model is verified. The proposed model can be used to analyze, plan, optimize, and design the information system to be constructed, and it helps to accelerate the informatization of manufacturing enterprises as well as promote the quality of their work.