Journal of South China University of Technology(Natural Science) >

2025 , Vol. 53 >Issue 8: 158 - 166

DOI: https://doi.org/10.12141/j.issn.1000-565X.250015

Materials Science & Technology

Preparation and Application of Modified Chitosan/Laponite Composite Antimicrobial Material

  • Lü Xiaojing ,
  • WEN Yuming ,
  • WANG Xiaoying ,
  • HU Jian
Expand
  • School of Light Industry and Engineering，South China University of Technology，Guangzhou 510640，Guangdong，China
吕晓静，女，博士生，工程师，主要从事日用化工材料及产品研究。E-mail： 201811007231@mail.scut.edu.cn

Received date: 2024-12-30

  Online published: 2025-03-10

Supported by

the Natural Science Foundation of Guangdong Province(2020B1515120038)

Fold

Abstract

Chitosan (CS) and laponite (LAP) are both biocompatible materials, and modification can enrich them with enhanced bioactivity. This paper investigates the preparation of a composite antibacterial material based on quaternized chitosan (QCS) and modified lithium montmorillonite, as well as its antibacterial performance in shampoo applications. QCS was degraded via microwave-assisted hydrogen peroxide oxidation to enhance its solubility, and LAP was organically modified with cetyltrimethylammonium bromide (CTAB) to impart its Malassezia adsorption capability, resulting in positively charged organic LAP. The successfully prepared QCS and CTAB-LAP composite materials were characterized and validated using Fourier-transform infrared spectroscopy, Zeta potential analysis, rotational rheometry, and scanning electron microscopy. Experimental results demonstrate d that QCS with different molecular weights exhibits good antibacterial performance in aqueous media, and the composite material of QCS and CTAB-modified LAP significantly enhances the antibacterial effect through synergistic action. By optimizing the dosage and ratio of the antibacterial agents, the study identified the optimal formulation of the composite antibacterial agent for use in both aqueous media and base shampoo. QCS of various molecular weights demonstrated excellent antimicrobial performance in aqueous media. The QCS and CTAB-LAP composite material exhibited a synergistic enhancement in antimicrobial effect. Optimal formulations for the composite antimicrobial agents in aqueous media and base shampoo were identified by varying the amount and ratio of the components. In aqueous solution, composite mixtures with mass ratios of 9∶1, 5∶5, and 1∶9 achieved 100% antimicrobial efficacy after 10-fold dilution and 5 minutes of Malassezia strain contact. When added to base shampoo at a 9∶1 ratio (total mass fraction 0.14%) and diluted 100-fold, the composite maintained significant antimicrobial efficacy, reaching up to 70% effectiveness compared to commercial chemical anti-dandruff products. This study highlights the potential of the QCS and CTAB-LAP composite for practical application in anti-dandruff shampoos and paves the way for the development of natural and eco-friendly antimicrobial materials.

Key words： quaternized chitosan; modified laponite; antimicrobial material; malassezia

Cite this article

Lü Xiaojing , WEN Yuming , WANG Xiaoying , HU Jian . Preparation and Application of Modified Chitosan/Laponite Composite Antimicrobial Material[J]. Journal of South China University of Technology(Natural Science), 2025 , 53(8) : 158 -166 . DOI: 10.12141/j.issn.1000-565X.250015

References

[1] FAERGEMANN J ．Malassezia yeasts and pityriasis versicolor［J］．Current Opinion in Infectious Diseases，2009，22（2）：128-133.
[2] HAY R J， GRAHAM-BROWN R A ．Dandruff and seborrhoeic dermatitis： causes and Management［J］． Clinical and Experimental Dermatology，1997，22（1）：3-6.
[3] RO B I， DAWSON T L ．The role of sebaceous gland activity and scalp microfloral metabolism in the etiology of dandruff［J］．Journal of Investigative Dermatology Symposium Proceedings，2005，10（3）：194-197.
[4] TURNER G A， HOPTROFF M， HARDING C R ．Stratum corneum dysfunction in dandruff［J］．International Journal of Cosmetic Science，2012，34（4）：298-306.
[5] ZHANG Y， ZHANG X， LIU M ．Malassezia species in seborrheic dermatitis and dandruff：a systematic review and meta-analysis［J］．Mycoses，2013，56（3）：267-275.
[6] GRICE E A， SEGRE J A ．The skin microbiome［J］． Nature Reviews Microbiology，2011，9（4）：244-253.
[7] DEANGELIS Y M， GEMMER C M， KACZVINSKY J R，et al ．Three etiologic factors in dandruff and seborrheic dermatitis：malassezia fungi，sebaceous lipids，and individual sensitivity［J］．Journal of Investigative Dermatology Symposium Proceedings，2005，10（3）：295-297.
[8] PONZIO R R， SURBER C ．Safety and efficacy of pyrithione zinc in the treatment of dandruff［J］．American Journal of Clinical Dermatology，2019，20（3）： 351-358.
[9] DRAELOS Z D ．The effect of antidandruff shampoos on hair quality［J］．International Journal of Cosmetic Science，2010，34（6）：455-459.
[10] MORAIS M G， FILHO P ．The role of natural ingredients in treating dandruff［J］．Journal of Cosmetic Dermatology，2005，14（2）：151-157.
[11] Commission European ．Commission Regulation （EU） 2021/1902 of amending Annexes II and V to Regulation （EC） No 1223/2009 as regards zinc pyrithione ［Z］．Luxembourg： Publications Office of the European Union，2021.
[12] LIU W R， YANG Y Y， LIU Y S ．Biocides in the river system of a highly urbanized region：a systematic investigation involving runoff input［J］．Science of the Total Environment，2018，624（5）：1023-1030.
[13] MUXIKA A， ETXABIDE A， URANGA J，et al ．Chitosan as a bioactive polymer：processing， properties and applications［J］．International Journal of Biological Macromolecules，2017，105（Pt2）：1358-1368.
[14] MOUZAHIM M EL， EDDRARAI E M， ELADAOUI S，et al ．Food packaging composite film based on chitosan，natural kaolinite clay，and Ficus carica leaves extract for fresh-cut apple slices preservation［J］．International Journal of Biological Macromolecules，2023，233：123430/1-11.
[15] TANPICHAI S， SRIMARUT Y， WORAPRAYOTE W，et al ．Chitosan coating for the preparation of multilayer coated paper for food-contact packaging：wettability，mechanical properties，and overall migration［J］．International Journal of Biological Macromolecules，2022，213（7）：534-545.
[16] AKBARI-ALAVIJEH S， SHADDEL R， JAFARI S M ． Encapsulation of food bioactives and nutraceuticals by various chitosan-based nanocarriers［J］．Food Hydrocolloids，2020，105：105774/1-11.
[17] XING X， HAN Y， CHENG H ．Biomedical applications of chitosan/silk fibroin composites：a review［J］．International Journal of Biological Macromolecules，2023，240：124407/1-20.
[18] AUGUSTINE R， REHMAN S R U， AHMED R，et al ．Electrospun chitosan membranes containing bioactive and therapeutic agents for enhanced wound healing［J］．International Journal of Biological Macromolecules，2020，156（8）：153-170.
[19] FENG P， LUO Y， KE C，et al ．Chitosan-based functional materials for skin wound repair： mechanisms and applications［J］．Frontiers in Bioengineering and Biotechnology，2021，9：650598/1-15.
[20] ZHAO R， MA T， ZHAO S，et al ．Uniform and stable immobilization of metal-organic frameworks into chitosan matrix for enhanced tetracycline removal from water［J］．Chemical Engineering Journal，2020，382：122893/1-11.
[21] QIU Y L， LI Y， ZHANG G L，et al ．Quaternary-ammonium chitosan， a promising packaging material in the food industry［J］．Carbohydrate Polymers，2024，323：121384/1-14.
[22] KIAEE G， DIMITRAKAKIS N， SHARIFZADEH S，et al ．Laponite-based nanomaterials for drug delivery［J］．Advanced Healthcare Materials，2022，11（7）：e2102054/1-39.
[23] MI Y， TAN W， ZHANG J，et al ．Modification of hydroxypropyltrimethyl ammonium chitosan with organic acid：synthesis， characterization，and antioxidant activity［J］．Polymers，2020，12（11）：2460/1-16.
[24] MOHAMMED M， SYEDA J， WASAN K M，et al ． An overview of chitosan nanoparticles and its application in non-parenteral drug delivery［J］．Pharmaceutics，2017，9（4）：53-60.
[25] ISLAM M M， MONDAL M I H， AHMED F ．Study on prawn shell waste into chitosan and its derivatives as value added products for cellulosic fibres［J］．Research Journal of Textile and Apparel，2017，21（2）：134-145.
[26] MA B， TAN W， ZHANG J，et al ．Preparation and characterization of chitosan derivatives bearing imidazole ring with antioxidant，antibacterial，and antifungal activities［J］．Starch-St?rke，2023，75（3/4）：2200204.
[27] YAO Y， YE Z， ZHANG Y，et al ．Quaternary ammonium compounds and their composites in antimicrobial applications［J］．Advanced Materials Interfaces，2024，11：2300946/1-19.
[28] FREITAS E D， MORAIS C F， JONAS K，et al ．An overview of current knowledge on the properties，synthesis and applications of quaternary chitosan derivatives［J］．Polymers，2020，12（12）：2878-2890.
[29] RABEA E I， BADAWY M E， STEVENS C V，et al ． Chitosan as antimicrobial agent：applications and mode of action［J］．Biomacromolecules，2003，4（6）：1457-1465.
[30] MCBAIN A J， LEDDER R G， SMITH T，et al ．Effects of quaternary-ammonium-based formulations on bacterial community dynamics and antimicrobial susceptibility［J］．Applied and Environmental Microbiology，2004，70（6）：3449-3456.
[31] TISCHER M， PRADEL G， OHLSEN K，et al ．Quaternary ammonium salts and their antimicrobial potential：targets or nonspecific interactions［J］． ChemMedChem，2012，7（1）：22-31.
Options
Outlines

/

〈 〉