Journal of South China University of Technology(Natural Science) >

2023 , Vol. 51 >Issue 12: 118 - 130

DOI: https://doi.org/10.12141/j.issn.1000-565X.220798

Food Science & Technology

Study on Beneficial Characteristics inVitro of Enterococcus Faecalis EF-ZA1107-06

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  • School of Food Science and Engineering，South China University of Technology，Guangzhou 510640，Guangdong，China
许喜林（1964-），男，博士，副教授，主要从事食品安全与控制研究。E-mail： xuxilin@scut.edu.cn

Received date: 2022-12-05

  Online published: 2023-06-20

Supported by

the National Natural Science Foundation of China(31771908)

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Abstract

Studies have shown that probiotic has good antidiabetic and antioxidant activities and great inhibitory effects on human cancer cell lines. This paper took α-glucosidase and α-amylase inhibition rate, superoxide anion radical scavenging rate, ferrous ion chelating rate and reducing activity as indexes, and compared the antidiabetic and antioxidant capacities in vitro between Enterococcus faecalis EF-ZA1107-06 and Lactobacillus rhamnosus GG (LGG). The effects of EF-ZA1107-06 on cellular oxidative stress were investigated by measuring the enzyme activities of glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD) and catalase (CAT) in Caco-2 cell lines. The anticancer activity and mechanism were also preliminarily explored using HepG2 and MDA-MB-231 cell lines. The results show that the supernatant of EF-ZA1107-06 has the highest scavenging rate of superoxide anion free radical from 40.78% to 59.61% and strongest ferrous ion chelating ability from 39.28% to 56.59%, while the lysate of EF-ZA1107-06 has the highest reducing activity, equivalent to 1.072 mmol/L equivalent cysteine, which is remarkably higher than that of LGG lysate (P<0.05). The inhibition of α-amylase and α-glucosidase activity confirms the antidiabetic activity of EF-ZA1107-06. Meanwhile, EF-ZA1107-06 can reduce oxidative damage caused by H2O2 to Caco-2 cells to a certain extent and inhibit the S phase and G2 phase of HepG2 cells, but only the S phase of MDA-MB-231 cells. The induction of the early apoptosis of HepG2 cells and MDA-MB-231 cells confirms the anticancer activity of EF-ZA1107-06.

Key words： Enterococcus faecalis; antioxidant capacity; anticancer activity; antidiabetic activity

Cite this article

XU Xilin, PENG Yunyan, ZHOU Xiaoli, et al . Study on Beneficial Characteristics inVitro of Enterococcus Faecalis EF-ZA1107-06[J]. Journal of South China University of Technology(Natural Science), 2023 , 51(12) : 118 -130 . DOI: 10.12141/j.issn.1000-565X.220798

References

1 刘洋，郭宇星，潘道东 ．4种乳酸菌体外抗氧化能力的比较研究［J］．食品科学，2012，33（11）：25-29.
  LIU Yang， GUO Yuxing， PANG Daodong ．Comparative antioxidant activity of four species of lactic acid bacteria in vitro ［J］．Food Science，2012，33（11）：25-29.
2 赵彤，钟宜科，荀一萍，等 ．乳酸菌抗氧化性及其作用机制研究进展［J］．中国食品添加剂，2018（9）：202-209.
  ZHAO Tong， ZHONG Yike， XUN Yiping，et al ．Research progress on antioxidation and regulation mechanism of lactic acid bacteria［J］．China Food Additives，2018（9）：202-209.
3 HAN W， ZHANG X L， WANG D W，et al ．Effects of microencapsulated Enterococcus fecalis CG1.0007 on growth performance，antioxidation activity，and intestinal microbiota in broiler chickens1［J］．Journal of Animal Science，2013，91（9）：4374-4382.
4 刘长建，刘秋，姜波，等 ．鹑鸡肠球菌m661的抗氧化、降胆固醇作用［J］．食品科学，2013，34（7）：157-161.
  LIU Changjian， LIU Qiu， JIANG Bo，et al ．Antioxidant and cholesterol-reducing properties of Enterococcus gallinarum m661［J］．Food Science，2013，34（7）：157-161.
5 董翎逸，刘正奇，裘梁，等 ．屎肠球菌WEFA23和海氏肠球菌WEHI01发酵荷叶上清的抑菌及抗氧化评价［J］．南昌大学学报（理科版），2018，42（4）：73-79.
  DONG Lingyi， LIU Zhengqi， QIU Liang，et al ．Antibacterial and antioxidant evaluation of supernatant of the fermented lotus leaf by Enterococcus faecium WETA23 and Enterococci hirae WHEHL01［J］．Journal of Nanchang University （Natural Science），2018，42（4）：73-79.
6 FRANZ C， STILES M E， SCHLEIGER K H，et al ． Enterococci in foods-a conundrum for food safety［J］．International Journal of Food Microbiology，2003，88（23）：105-122.
7 WOO J Y， GU W， KIM K A，et al ． Lactobacillus pentosus var. plantarum C29 ameliorates memory impairment and inflammaging in a D-galactose-induced accelerated aging mouse model ［J］．Anaerobe，2014，27：22-26.
8 THIRABUNYANON M， BOONPRASOM P， NIAMSUP P ．Probiotic potential of lactic acid bacteria isolated from fermented dairy milks on antiproliferation of colon cancer cells［J］．Biotechnology Letters，2009，31（4）：571-576.
9 CHOUDHURI I， KHANRA K， PARIVA P，et al ．Structural characterization of an exopolysaccharide isolated from Enterococcus faecalis，and study on its antioxidant activity，and cytotoxicity against HeLa cells［J］．Current Microbiology，2020，77（10）：3125-3135.
10 AMER M N， MANSOUR N M， El-DIWANY A I，et al ．Isolation of probiotic Lactobacilli strains harboring L-asparaginase and arginine deiminase genes from human infant feces for their potential application in cancer prevention［J］．Annls of Microbiology，2013，63（3）：1121-1129.
11 WANG Y， WATERS J， LEUNG M L，et al ．Clonal evolution in breast cancer revealed by single nucleus genome sequencing［J］．Nature，2014，512（7513）：155-160.
12 RAWLA P， SUNKARA T， BARSOUK A. Epidemiology of colorectal cancer：incidence，mortality，survival，and risk factors［J］．Przeglad Gastroenterologiczny，2019，14（2）：89-103.
13 UCCELLO M， MALAGUARNERA G， BASILE F，et al ．Potential role of probiotics on colorectal cancer prevention［J］．BMC Surgery，2012，12：S35/1-8.
14 CASTRO M S， MOLINA M A， SCIULLO P D，et al ．Beneficial activity of Enterococcus faecalis CECT7121 in the anti-lymphoma protective response［J］．Journal of Applied Microbiology，2010，109（4）：1234-1243.
15 PANWAR H， CALDERWOOD D， GRANT I R，et al ． Lactobacillus strains isolated from infant faeces possess potent inhibitory activity against intestinal alpha-and beta-glucosidases suggesting antidiabetic potential［J］．European Journal of Nutrition，2014，53（7）：1465-1474.
16 周晓莉 ．粪肠球菌EF-ZA1107-06的益生性及高密度培养的研究［D］．广州：华南理工大学，2021.
17 YADAV H， JAIN S， SINHA P R ．Antidiabetic effect of probiotic dahi containing Lactobacillus acidophilus and Lactobacillus casei in high fructose fed rats［J］．Nutrition，2007，23（1）：62-68.
18 刘顺，谢远红，张红星，等 ．具有潜在降血糖作用乳酸菌的筛选［J］．食品工业科技，2019，40（16）：255-259，273.
  LIU Shun， XIE Yuanhong， ZHANG Hongxing，et al ．Screening of lactic acid bacteria with potential hypoglycemic effect［J］．Science and Technology of Food Industry，2019，40（16）：255-259，273.
19 孙明雪，邹纯才，鄢海燕 ．瓜蒌提取物抗氧化药效成分群的筛选与验证［J］．南方医科大学学报，2022，42（3）：384-391.
  SUN Mingxue， ZOU Chuncai， YAN Haiyan ．Screening of effective antioxidant components from Trichosanthes extract and assessment of their antioxidant activity［J］．Journal of Southern Medical University，2022，42（3）：384-391.
20 束文秀，吴祖芳，翁佩芳，等 ．植物乳杆菌和发酵乳杆菌对胡柚汁发酵品质及其抗氧化性的影响［J］．食品科学，2019，40（2）：152-158.
  SHU Wenxiu， WU Zufang， WENG Peifang，et al ．Comparison of quality characteristics and antioxidant activity of the fruit juice of Citrus paradisi cv．changshan huyou fermented by Lactobacillus plantarum and Lactobacillus fermentym ［J］．Food Science，2019，40（2）：152-158.
21 LIN M Y， YEN C L ．Antioxidative ability of lactic acid bacteria［J］．Journal of Agricultural and Food Chemistry，1999，47（4）：1460-1466.
22 DAS D， GOYAL A ．Antioxidant activity and γ-aminobutyric acid （GABA） producing ability of probiotic Lactobacillus plantarum DM5 isolated from Marcha of Sikkim［J］．LWT-Food Science and Technology，2015，61（1）：263-268.
23 KULLISAAR T， ZILMER M， MIKELSAAR M，et al ．Two antioxidative lactobacilli strains as promising probiotics［J］．International Journal of Food Microbiology，2002，72（3）：215-224.
24 刘少敏 ．不同乳酸菌抗氧化能力比较及其机制的研究［D］．哈尔滨：东北农业大学，2015.
25 SHEN W， SONG D， WU J，et al ．Protective effect of a polysaccharide isolated from a cultivated Cordyceps mycelia on hydrogen peroxide-induced oxidative damage in PC12 cells［J］．Phytotherapy Research，2011，25（5）：675-680.
26 张园莲 ．抑制结直肠癌细胞HT-29增殖的益生菌筛选及其作用机理研究［D］．昆明：昆明理工大学，2018.
27 王淑梅 ．抗结肠癌功能益生菌筛选及其诱导HT-29细胞凋亡机制研究［D］．哈尔滨：哈尔滨工业大学，2014.
28 ZHANG J F， ZHENG Y G， SHEN Y C ．Inhibitory effect of valienamine on the enzymatic activity of honeybee （Apis cerana Fabr.） α-glucosidase［J］．Pesticide Biochemistry & Physiology，2007，87（1）：73-77.
29 ZENG Z， LUO J Y， ZUO F，et al ．Screening for potential novel probiotic Lactobacillus strains based on high dipeptidyl peptidase IV and α-glucosidase inhibitory activity［J］．Journal of Functional Foods，2016，20：486-495.
30 龙楚媚，付强，王琪，等 ．罗汉果内生菌的分离及α-淀粉酶抑制剂产生菌的筛选［J］．中国酿造，2019，38（1）：71-74.
  LONG Chumei， FU Qiang， WANG Qi，et al ．Isolation of endophytes from Siraitia grosvenorii and screening of α-amylase inhibitors-producing strains ［J］．China Brewing，2019，38（1）：71-74.
31 RAMCHANDRAN L， SHAH N P ．Effect of exopolysaccharides and inulin on the proteolytic，angiotensin-I-converting enzyme- and α-glucosidase-inhibitory activities as well as on textural and rheological properties of low-fat yogurt during refrigerated storage［J］．Dairy Science & Technology，2009，89（6）：583-600.
32 张江巍，曹郁生，李海星，等 ．乳酸菌抗氧化活性及检测方法［J］．中国乳品工业，2005，33（9）：53-55.
  ZHANG Jiangwei， CAO Yusheng， LI Haixing，et al ．Antioxidative activities of lactic acid bacteria and the test method［J］．China Dairy Industry，2005，33（9）：53-55.
33 巩蕾，都启晶，赵宏坤 ．屎肠球菌JT_1的抗氧化特性［J］．食品科技，2017，42（6）：30-35.
  GONG Lei， DU Qijing， ZHAO Hongkun ．The oxidation resistance of Enterococcus faecium JT1［J］．Food Science and Technology，2017，42（6）：30-35.
34 郭慧芬 ．发酵肉制品中抗氧化乳酸菌的筛选及其特性的初步研究［D］．呼和浩特：内蒙古农业大学，2017.
35 HUANG G J， CHEN H J， CHANG Y S，et al ．Recombinant sporamin and its synthesized peptides with antioxidant activities in vitro ［J］．Botanical Studies，2007，48（2）：133-140.
36 吴贝，张盼望，王喜亮，等 ．益生屎肠球菌HDRsEf1抗氧化性能评估［J］．华中农业大学学报，2016，35（6）：98-103.
  WU Bei， ZHANG Panwang， WANG Xiliang，et al ．Antioxidant performance evaluation of Enterococcus faecium HDRsEf1［J］．Journal of Huazhong Agricultural University，2016，35（6）：98-103.
37 张书文 ．抗氧化乳酸菌的筛选及其特性研究［D］．呼和浩特：内蒙古农业大学，2009.
38 陈佩，党辉 ．干酪乳杆菌对HepG2细胞抗氧化功能的影响［J］．食品科学，2017，38（11）：220-224.
  CHEN Pei， DANG Hui ．Effect of Lactobacillus casei on antioxidant function of HepG2 cells［J］．Food Science，2017，38（11）：220-224.
39 LIN M Y， CHANG F J ．Antioxidative effect of intestinal bacteria Bifidobacterium longum ATCC 15708 and Lactobacillus acidophilus ATCC 4356［J］．Digestive Diseases & Sciences，2000，45（8）：1617-1622.
40 崔志文，黄琴，黄怡，等 ．鼠李糖乳酸杆菌对Caco-2细胞抗氧化功能的影响［J］．中国农业科学，2011，44（23）：4926-4932.
  CUI Zhiwen， HUANG Qin， HUANG Yi，et al ．Antioxidative function of Lacbacillus rhamnosus to Caco-2 cells［J］．Scientia Agricultura Sinica，2011，44（23）：4926-4932.
41 HASSAN Z， MUSTAFA S， RAHIM R A，et al ．Anti-breast cancer effects of live，heat-killed and cytoplasmic fractions of Enterococcus faecalis and Staphylococcus hominis isolated from human breast milk［J］．In Vitro Cellular & Developmental Biology-Animal，2016，52（3）：337-348.
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