Journal of South China University of Technology(Natural Science) >

2023 , Vol. 51 >Issue 1: 69 - 75

DOI: https://doi.org/10.12141/j.issn.1000-565X.220062

Materials Science & Technology

Preparation and Properties of Fibrin Gel Tubular Scaffold

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  • 1.School of Biology and Biological Engineering，South China University of Technology，Guangzhou 510006，Guangdong，China
    2.School of Medicine，South China University of Technology，Guangzhou 510006，Guangdong，China
    3.Department of Cardiology，Guangdong Provincial People’s Hospital （Guangdong Academy of Medical Sciences），Guangzhou 510080，Guangdong，China
    4.Guangdong Provincial Geriatrics Institute，Guangzhou 510080，Guangdong，China
林展翼（1965-），男，博士，教授，主要从事组织工程和生物医学工程研究。

Received date: 2022-02-17

  Online published: 2022-05-05

Supported by

the “Top Plan” Project of High Level Hospital of Guangdong Province(DFJH201904);the Research and Development Project in Key Areas of Guangdong Province(2016B070701007)

Fold

Abstract

Suitable scaffold material is one of the keys to the successful construction of small caliber tissue engineering blood vessels in vitro. Fibrin gel with natural protein composition is an ideal source of scaffold materials. In this study, fibrinogen was used as raw material and mixed with a certain proportion of thrombin and calcium chloride, and the gel material was obtained through technical steps such as temperature control. Then, the clotting time, water absorption, degradation time and mechanical properties were tested. The microstructure of the material was observed by scanning electron microscope. Secondly, the hydrogel tubular scaffold was made by using a specific tubular mold. By loading human fibroblasts in the scaffold, the effect of fibrin gel scaffold on the growth of human fibroblasts was studied and its cytocompatibility was judged. The results show that the overall appearance of the fibrin gel obtained by this method is milky white, smooth and uniform in thickness; the average gel forming time is (172.0±4.7) s; the water absorption rate is 34.50%±1.87%; the complete degradation time is 7 days and the Young's modulus is (2624±295) Pa; the overall structure of the gel has certain stability; the microstructure of the gel is presented as porous mesh fibers, and the fiber diameter is (0.41±0.03) μm with average pore size of reticular fiber of (47.87±9.60) μm2. After embedding human fibroblasts, the cells are morphologically intact and evenly distributed in the gel, and the cells survived well. This study provides a reference for the further study of in vitro construction of small caliber tissue engineering vascular graft.

Key words： fibrin gel; human fibroblast; porous tubular scaffold; vascular graft

Cite this article

LIN Zhanyi, LIU Peng, MEI Jingyi, et al . Preparation and Properties of Fibrin Gel Tubular Scaffold[J]. Journal of South China University of Technology(Natural Science), 2023 , 51(1) : 69 -75 . DOI: 10.12141/j.issn.1000-565X.220062

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