Molecular models of base asphalt，bio-oil，and bio-asphalt were constructed based on molecular dynamics in order to study the compatibility of wood chip based bio-oil and asphalt，and the compatibility of bio-oil with asphalt and its components was quantitatively analyzed by molecular polarity，solubility parameters，and binding energy.The simulation results were validated with the help of the segregation test.The results show that the solubility parameters of bio-oil are closer to those of asphaltene fraction，both of them having stronger polarities and more susceptible to adsorption.As the temperature increases，the difference in solubility parameters between bio-oil and asphalt gradually becomes larger and the binding energy gradually decreases.The difference of parameters in solubility and the binding energy significantly deteriorates when the system temperature exceeds 433K，and the storage stability of bio-asphalt decreases obviously when the temperature reaches 453K.To ensure the compatibility between bio-oil and asphalt and avoid serious segregation during high-temperature storage，it is recommended that the storage temperature of bio-asphalt should not exceed 433K.