Glucose and octenylsuccinate anhydride (OSA) were modified by esterification. The synthetic octenylsuccinate glucose ester (OSGE) was separated and purified to prepare OSGE with a sugar ester content of about 50%. Its irritation or interfacial properties were studied by taking linear sodium dodecylbenzene sulfonate (LAS), alkyl polyglucoside (APG) and cocamidopropyl betaine (CAB) as control group. Results show that the surface tension of OSGE and LAS is about 28mN/m; the critical micelle concentration (CMC) of surfactant follows the order: OSGE < LAS < APG < CAB; the hydrophile-lipophile balance (HLB) of OSGE is 16.90, indicating its highest hydrophilic property. No significant difference was found among OSGE, LAS and APG in emulsification ability, while irritation of OSGE is much lower than that of other three surfactants.