In order to study the fire resistance of the cross-laminated timber (CLT) wall made of domestic Japanese larch, the CLT plate normal temperature material performance test and the CLT wall fire resistance test under the standard heating curve were carried out. The performance test obtained the interlayer shear strength, rolling shear strength and compressive strength along the grain of the CLT sheet. The fire resistance test results show that the failure mode of the wall at room temperature is mainly buckling failure, and the ultimate load of the three-layer CLT wall is higher than that of the five-layer CLT wall. Under the same layer composition, the fire resistance of the CLT wall decreases with the increase of the load-holding ratio of the test piece. Under the same load-holding ratio, the fire resistance of the five-layer CLT wall is higher than that of the three-layer CLT wall. Moreover, the CLT wall did not fall off during the test. The numerical simulation with ABAQUS shows that the temperature field and displacement development trends obtained are basically consistent with the test results.