In this study，the acute toxicological safety，prebiotic in vitro and functional characteristics in vivo of Bacillus coagulans 13002 were evaluated.The results show that Bacillus coagulans 13002 is non-toxic，which acutely oral for female and male KM mice，and that it possesses good tolerance to simulated gastrointestinal digestion fluid.Moreover，it is resistant to Staphylococcus aureus ATCC 12598 （G+） and Escherichia coli ATCC 25922 （G-），which can obviously destroy the microstructure.These results indicate that the combination of Bacillus coagulans 13002 with fructooligosaccharide has a certain recovery effect on cyclophosphamide-induced tissue damage in mice，which can increase blood cells，improve protein synthesis rate and glucose utilization，and remove excess cholesterol to maintain the health of mice.