Based on RNG k-ε turbulent model，, the internal flow field of a vane oil pump with different vanes spacing was simulated by computational fluid dynamics software，t. The steady flow characteristic curve was obtained. And it is and compared with the experimental results. Considered the effectinfluences of different vanes spacing configuration，, the pressure pulsation at each monitoring points of internal flow field was analyzed in frequency domain. The results show that，compared with the even vane spacing configuration of even vanes spacing，the energy amplitude of pressure pulsation in pump outlet and oil pressure cavity decreases at the fundamental frequency multiple frequency，, and and decreases obviously at the third harmonic wave. The pressure pulsation and noise test analysis awere carried out on the experimental prototype. The test results show that，, compared with the even vane spacing configuration, the uneven vanes spacing configuration can reduce the pressure pulsation of the vane oil pump and the noise value of third and fourth harmonic is reducing by wave comparing with the regular vanes spacing configuration.