Highly efficient asymmetric oxidation of racemic 1-phenyl-1，2-ethanediol ( PED) was successfully con- ducted to prepare enantiomerically pure ( S) -PED with immobilized Kurthia gibsonii SC0312 cells in non-aqueous system containing DESs． Among the 4 kind of tested ionic liquids and 2 kind of deep eutectic solvents，［ChCl］［U］ manifested better biocompatibility with Kurthia gibsonii SC0312 cells，and it can appropriately change the cell membrane integrity，thereby effectively improve the catalytic efficiency of this reaction． When ［ChCl］［U］content is 4% ( volume fraction) ，buffer pH is 7． 5，reaction temperature is 35 ℃，cell concentration is 60 mg /mL，and substrate concentration is 100 mmol /L，the initial reaction rate reaches 8． 25 mmol /( L·h) ，and after 7 hours' re- action，the yield rate and ( S) -PED e． e． value reach 49． 6% and 99． 9% ，respectively．