In order to implement the high-valued utilization of tobacco stem,supercritical CO₂ fluid extraction was conducted to extract the flavor components from waste tobacco stem and to prepare essential oil with natural tobacco flavors.Then,by taking the extraction yield as an evaluation index,single-factor and orthogonal experiments were respectively carried out to optimize the extraction process,Finally,the flavor components in the obtained extracts were analyzed by means of GC-MS.The results show that,under the optimal extraction conditions,that is,an ex- traction temperature of 60 ℃,an extraction pressure of 32 MPa,a CO₂ flow rate of 24 L/h,an extraction time of 2. 5 h,when 85% ethanol solution with a mass fraction in tobacco stem of 20% is adopted as the entrainer,the ex- traction yield reaches 1. 70%; and that many important flavor components are contained in the 27 kinds of identi- fied components,such as β-damascenone,megastigmatrienone,β-cembrenediol,geranyl acetone,neophytadiene,and so on.