Aiming at the location and construction sequence approach of exclusive urban bus lanes in a certain planning period,this paper analyzes the location and construction practice as well as the dynamic change of realistic demands,and establishes a time-dependent bi-level optimization model to locate exclusive bus lanes.The upper level of the proposed model is a locating model to maximize the total consumer surplus in the whole road network,and the lower level is an allocation model of cars and buses to minimize the generalized travel cost.Then,with the help of genetic algorithm,the mentioned problem is solved.Moreover,a case study is performed to verify the effectiveness and practicability of the established bi-level model and to reveal the superiority of the proposed approach considering time dimensionality to the simply-phased sequential approach.The results show that the established model can simultaneously optimize the location and construction sequence of exclusive bus lanes.