The chemical structure of the enzymatic hydrolysis lignin ( EHL) from corn stover was characterized by means of UV spectroscopy,infrared spectroscopy,elemental analysis,headspace gas chromatography and potentiometric titration.It is found that there exist more substituents in the aromatic ring of the EHL,and β-O-4 linkage is of lower fracture degree,and that,the main structural unit was H-type,and the methoxy group,phenolic hydroxyl group and carboxyl contents of the EHL are respectively 6.50%,0.48% and 2.46%.By mixing the lignin and the epoxy resin to prepare the EHL /epoxy resin composite,it can be concluded that the EHL within a certain dosage can improve the tensile strength and bending strength of the EHL /epoxy resin composite,and the heat resistance of the EHL /epoxy resin composite increases with the EHL dosage,and that at 460℃,the residual amount of the epoxy resin composite containing 2.0% EHL is 8.3%,while the residual amount of pure epoxy resin is only 4.4%.The above-mentioned results show that the EHL can improve the mechanical properties and heat resistance of epoxy resin significantly,so it has a good application prospect in epoxy resin composites.