The peroxidation of unsaturated fatty acid caused by lipoxygenase (LOX) is known to be responsible for the deterioration of storage quality in maize(Zea mays L.) seed.In order to inhibit the expression of key aging en- zyme Zmlox-2 gene in maize,Zmlox-2 gene in maize seed was cloned according to the principle of RNA interfer- ence (RNAi),and the RNAi plant expression vector containing Zmlox-2 was constructed by means of recombinant PCR and enzyme connection technology.Then,the recombinant vector pRI101-on-Zmlox-2-RNAi was transformed into Agrobacterium tumefaciens via heat shock method.Experimental results show that the RNAi expression vector containing Zmlox-2 gene has been successfully constructed.This research lays a theoretical and experimental foun- dation for the induction of genetic transformation and the exploration of interference expression effect of aging en- zyme gene.