A medical bacterial cellulose medium with simple and definite components waswere obtained by adding trace elements with coconut juice as the basic carbon source and the growth factor source, yeast extract as the nitrogen source and ethanol as the addition carbon source. The medium contains components with relatively stable content and can improve the fermentation rate and the yield of bacterial cellulose, and the product thickness can meet various medical requirements. Then, alkali is used to purify the fermentation product by combining SDS or ultrasound or ethanol. The results show that alkali and SDS treatment procedure has the best purification effect, and that the purified sample possesses a content of impurity nitrogen below the detection limit and a high transmittance which is close to cornea in vivo in the visible region, and it exhibits a good biocompatibility  withto L929 cells.