Construction of RNA Interference Expression Vector Containing Key Aging Enzyme Zmlox-2 in Zea Mays

doi:10.3969/j.issn.1000-565X.2016.03.019

Journal of South China University of Technology (Natural Science Edition) ›› 2016, Vol. 44 ›› Issue (3): 136-141.doi: 10.3969/j.issn.1000-565X.2016.03.019

• Biological Engineering • Previous Articles Next Articles

Construction of RNA Interference Expression Vector Containing Key Aging Enzyme Zmlox-2 in Zea Mays

HAN Ying¹ ZHAO Shou-jing¹ YANG Yu¹ SUN Yao² WANG Le¹

1.College of Biological and Agricultural Engineering,Jilin University,Changchun 130022,Jilin,China; 2.College of Chemistry and Life Science,Changchun University of Technology,Changchun 130012,Jilin,China

Received:2015-04-15 Revised:2015-06-29 Online:2016-03-25 Published:2016-02-02
Contact: 赵寿经(1961-)，男，教授，主要从事农业生物环境与能源工程研究． E-mail:swgc@jlu.edu.cn
About author:韩颖(1971-)，女，博士生，副教授，主要从事农业生物环境与能源工程研究． E-mail:jlgshy@163． com
Supported by:
Supported by the Jilin Scientific and Technological Development Program(20130102041JC)

Abstract

Abstract: The peroxidation of unsaturated fatty acid caused by lipoxygenase (LOX) is known to be responsible for the deterioration of storage quality in maize(Zea mays L.) seed.In order to inhibit the expression of key aging en- zyme Zmlox-2 gene in maize,Zmlox-2 gene in maize seed was cloned according to the principle of RNA interfer- ence (RNAi),and the RNAi plant expression vector containing Zmlox-2 was constructed by means of recombinant PCR and enzyme connection technology.Then,the recombinant vector pRI101-on-Zmlox-2-RNAi was transformed into Agrobacterium tumefaciens via heat shock method.Experimental results show that the RNAi expression vector containing Zmlox-2 gene has been successfully constructed.This research lays a theoretical and experimental foun- dation for the induction of genetic transformation and the exploration of interference expression effect of aging en- zyme gene.

Key words: bioengineering, aging, RNA interference expression vector, recombinant PCR, lipoxygenase

CLC Number:

HAN Ying ZHAO Shou-jing YANG Yu SUN Yao WANG Le. Construction of RNA Interference Expression Vector Containing Key Aging Enzyme Zmlox-2 in Zea Mays[J]. Journal of South China University of Technology (Natural Science Edition), 2016, 44(3): 136-141.

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Construction of RNA Interference Expression Vector Containing Key Aging Enzyme Zmlox-2 in Zea Mays

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