Journal of South China University of Technology (Natural Science Edition) ›› 2015, Vol. 43 ›› Issue (2): 8-12.doi: 10.3969/j.issn.1000-565X.2015.02.002

• Food Science & Technology • Previous Articles     Next Articles

Determination of 4-Methylimidazole Content via Ion Chromatography-Mass Spectrometry

Yu Shu-juan Liu Ding-bo Xu Xian-bing Wu Xin-lan   

  1. School of Light Industry and Food Sciences，South China University of Technology，Guangzhou 510640，Guangdong，China
  • Received:2014-05-21 Revised:2014-07-10 Online:2015-02-25 Published:2014-12-31
  • Contact: 于淑娟(1955-)，女，教授，博士生导师，主要从事制糖工程、碳水化合物分子修饰的研究． E-mail:lfshjyu@scut.edu.cn
  • About author:于淑娟(1955-)，女，教授，博士生导师，主要从事制糖工程、碳水化合物分子修饰的研究．
  • Supported by:
    Supported by the National Natural Science Foundation of China(31271889)and the Science and Technology Pro-ject Item of Guangdong Province(2014B020205001)

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Abstract: In order to quantitatively and qualitatively determine the content of 4-methylimidazole in samples with simplicity，convenience and accuracy，a new method using cation exchange chromatography-mass spectrometry was developed． This method，with a detection limit of 0． 25μg/L，a linear range varying from 2． 5 to 200μg/L，a linear correlation coefficient of 0． 9997，a relative standard deviation of 2． 32% ～ 4． 25% and a recovery varying from 92． 7% to 96． 3%，was then used to test the 4-methylimidazole in caramel pigment，cola and some other samples．The results show that the proposed method is of high separation degree，good sensitivity，accurate quantification，high precision and low detection limit．

Key words: ion chromatography-mass spectrometry, 4-methylimidazole, caramel pigment, Maillard reaction, sen-sitivity, detection limit

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