Journal of South China University of Technology (Natural Science Edition) ›› 2005, Vol. 33 ›› Issue (10): 84-89.

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Interaction Between a New Antibiotic W anlongmycin and M etal Ions

Gao Xiang-yang1  Lin Bi-run2  Yao Ru-hua1  Chen Nian3   

  1. 1.College of Biological Science and Engineering,South China Univ.of Tech.,Guangzhou 5 10640,Guangdong,China;2·Plant Protection Research Institute,Guangdong Academy of Agricultural Sciences, Guangzhou 510640,Guangdong.China;3.College of Life Science,South China Agricultural Univ., Guangzhou 5 10642,Guangdong,China
  • Received:2004-12-05 Online:2005-10-25 Published:2005-10-25
  • Contact: 高向阳(1966-)，女，在职博士生，华南农业大学生命科学学院副教授，主要从事生物化学与生物制药方面的研究 E-mail:gaogxy@yahoo.corn.cn
  • About author:高向阳(1966-)，女，在职博士生，华南农业大学生命科学学院副教授，主要从事生物化学与生物制药方面的研究
  • Supported by:

    国家“863”高科技计划资助项目(2001 AA-246015)；国家自然科学基金资助项目(30471161)；广东省自然科学基金团队资助项目(2KB042051)；广东省自然科学基金资助项目(032011)

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Abstract:

The ultraviolet(UV)and fluorescent spectra of Wanlongmycin were first investigated.with a relatively strong UV absorbance occurring within the range of 1 90 to 300 nm in alcohol solvent. and two highest peaks located at the wavelengths of 202 nm and 228 nm.Then.the wavelengths of 290 nm and 330 nm were respectivelv selected as the optimum fluorescent excitation and emission wavelengths to study the interaction between Wanlongmycin and metal ions.The results indicate that,in the presence of Ca2+, Al3+ ,Cu2+ and Mg2+ ,a decrease in peak intensity and a violet shift of the UV spectrum of Wanlongmycin, as well as an obvious fluorescent quenching effect,can be observed,which means that Wanlongmycin can interact with these metal ions to form a stable Wanlongmycin-metal ion complex. Moreover,it is indicated that Zn2+ with different concentrations hardly influences the UV spectrum,the relative fluorescent intensity and the location of the peaks. which means that there is no interacti0n between Wanlongmycin and Zn2+.

Key words: Wanlongmycin, metal ion, ultraviolet spectrum, fluorescent spectrum