采用荧光光谱矩阵( EEM) 法探讨了铜绿微囊藻胞内外溶解性有机物的荧光特征，并结合蓝藻生化指标( 总蛋白含量、藻蓝蛋白含量、叶绿素a 含量以及藻毒素( MCLR)含量) 研究了氯化和短波紫外线照射( UVC) 处理铜绿微囊藻的机理．结果表明，与铜绿微囊藻相关的EEM 荧光物质主要有类蛋白质物质( 位于峰A 和B) 与类腐殖质物质( 位于峰C 和D) ．UVC 的灭活机理主要包括光降解和高级氧化，UVC 处理60 min 后胞内类蛋白质物质被降解并转化为胞外类腐殖质物质，120 min 后残留较少; 30 min 内藻蓝蛋白和MC-LR 的去除率分别达到83. 55%和94. 70%，总蛋白和叶绿素a 去除率较低．氯化处理主要依靠HClO 穿透进入藻细胞内部进行化学破坏和降解，处理60 min 后胞内外类蛋白质物质减少，而胞外类腐殖质物质增加且有明显的残留; 30min 内藻蓝蛋白和总蛋白去除率分别达到96. 44%和60. 36%，MC-LR 和叶绿素a 去除率较低．比较发现: 中等强度UVC 灭藻及有机物降解效果显著; HClO 能有效灭藻，但对水中溶解性有害有机物的控制效果不佳．

In this paper,the fluorescent characteristics of intracellular and extracellular dissolved organic matters of Microcystis aeruginosa were investigated by means of the EEM ( Excitation Emission Matrix) fluorescence spectroscopy,and the inactivation mechanism of Microcystis aeruginosa by chlorination and UVC irradiation was analyzed according to the cyanobacterial biochemical indexes such as the contents of total protein,phycocyanin,chlorophylla
and microcystin-LR. The results indicate that ( 1) the characteristic materials associated with Microcystis aeruginosa are mainly protein-like matters corresponding to peaks A and B and humic-like matters corresponding to peaks C and D; ( 2) the inactivation of Microcystis aeruginosa by UVC irradiation is mainly due to the photodegradation and advanced oxidation; ( 3) after a UVC irradiation for 60 min,the intracellular protein-like matters are transformed into extracellular humic-like ones,and few residuals are found after 120min; ( 4) the removals of phycocyanin and microcystin-LR respectively reach 83.55% and 94.70% after 30 min,while the removals of total protein and chlorophyll-a are relatively low; ( 5) the inactivation of Microcystis aeruginosa by chlorination is mainly due to the chemical ablation and degradation of cyanobacteria cells by the permeated HClO; ( 6) after a chlorination for 60 min,both the intracellular and the extracellular protein-like matters decline with significant remain of extracellular humic-like matters; ( 7) the removals of phycocyanin and total protein respectively reach 96. 44% and 60. 36% after 30min,while the removals of microcystin-LR and chlorophyll-a are relatively low; ( 8) the UVC irradiation with medium intensity is effective in the removal of cyanobacteria and the purification of dissolved organic matters; and ( 9) the HClO permeated into cyanobacteria cells is also effective in the removal of cyanobacteria but is ineffective
in the purification of dissolved organic matters.