对凝结芽孢杆菌（Bacillus coagulans）13002进行急性毒理安全性评价及体外益生性、体内功能特性研究，发现：凝结芽孢杆菌13002对雌雄性KM小鼠经口急毒实验，属无毒级，且对模拟胃肠道消化液具有良好的耐受性；对金黄色葡萄球菌ATCC 12598（G+）和大肠杆菌ATCC 25922（G-）均具有一定的抑菌能力，可明显破坏菌体的微观结构。结果表明，凝结芽孢杆菌13002与低聚果糖合用时，对环磷酰胺所致的小鼠机体组织损伤有一定的恢复作用，可在一定程度上增大血液中的细胞含量，提高蛋白质合成率、葡萄糖利用率，清除小鼠机体多余的胆固醇，维护小鼠机体健康。

In this study，the acute toxicological safety，prebiotic in vitro and functional characteristics in vivo of Bacillus coagulans 13002 were evaluated.The results show that Bacillus coagulans 13002 is non-toxic，which acutely oral for female and male KM mice，and that it possesses good tolerance to simulated gastrointestinal digestion fluid.Moreover，it is resistant to Staphylococcus aureus ATCC 12598 （G+） and Escherichia coli ATCC 25922 （G-），which can obviously destroy the microstructure.These results indicate that the combination of Bacillus coagulans 13002 with fructooligosaccharide has a certain recovery effect on cyclophosphamide-induced tissue damage in mice，which can increase blood cells，improve protein synthesis rate and glucose utilization，and remove excess cholesterol to maintain the health of mice.