以多重耐药金黄色葡萄球菌为研究对象，通过测定非抗生素类药物舍曲林与不 同抗生素作用的最低抑菌浓度、生长速率、时间 － 杀菌曲线及实时荧光定量 PCＲ 定量检 测耐药基因 mecA 和外排泵基因 norA 的转录水平，探讨舍曲林对金黄色葡萄球菌多重耐 药性的逆转作用及逆转机制． 结果显示:当舍曲林质量浓度高于 1 μg/mL 时，对金黄色葡 萄球菌有抑制作用;亚抑菌浓度(1/2 MIC)的舍曲林和四环素联用时对金黄色葡萄球菌 具有杀菌作用;当舍曲林和四环素、环丙沙星、克林霉素联用时对金黄色葡萄球菌具有协 同或相加抑制作用，可使其对 3 种抗生素的敏感性提高 75% 以上;低质量浓度的舍曲林 可显著降低外排泵基因 norA 和 SCCmec 基因盒中 mecA 基因的表达水平． 实验结果表明， 舍曲林能通过抑制 norA 和 mecA 等耐药基因的表达来调控金黄色葡萄球菌的多重耐药 性，通过联合抑菌部分逆转金黄色葡萄球菌对多种抗生素的耐药性．

Multidrug-resistant Staphylococcus aureus (S. aureus) were taken as object to investigate the reversal effect of sertraline on multi-drug resistance of S. aureus and its reversal mechanism through the determination of minimum inhibitory concentration，growth rate，time-kill curves under the combination of non-antibiotics sertraline and different antibiotics，as well as transcriptional level of the drug resistance gene mecA and the efflux pump gene norA by real-time PCR． The results indicate that when the sertraline concentration is higher than 1 μg/mL，the growths of S. aureus are inhibited; when sertraline (1/2 MIC) is combined with tetracycline，it has a bactericidal effect on S. aureus． Sertraline combined with tetracycline，ciprofloxacin，and clindamycin，it has synergistic or additive inhibitory effect on S. aureus，which could increase the sensitivity of three antibiotics by more than 75%． Low concentration of sertraline could significantly reduce the expression of the efflux pump gene norA and mecA gene in SCCmec gene cassettes of S. aureus． The results show that sertraline can regulate the multi-drug resistance of S. aureus by inhibiting the expression of resistance genes such as norA and mecA，and reverse the resistance of S. aureus to various antibiotics when it is combined with antibiotics．