通过测定清除DPPH 自由基的活性和氧自由基吸收能力实验评价不同蛋白酶酶解河蚬( Corbicula fluminea) 所得酶解物的抗氧化性; 运用MTT 检测法和检测谷丙转氨酶、 谷草转氨酶活性研究不同乙醇浓度对LO2 细胞的影响，并评价河蚬酶解物对乙醇致LO2 细胞损伤的保护作用; 最后对活性高的样品进行了氨基酸分析．结果显示: 河蚬酶解物具备一定的抗氧化能力，河蚬酶解物( 300 ～400mg/L) 对 LO2 细胞的增殖无毒性，且可以抑制乙醇诱导降低 LO2 细胞存活率的趋势，其中胰酶和复合风味蛋白酶酶解物的保护作用显著优于其他酶解物，同时二者可减缓乙醇导致的LO2 细胞 ALT 和 AST 的释放，各自含对抗氧化有贡献的氨基酸占其总氨基酸的比例分别是75．69%、75．06%．

The antioxidant activity of the Corbicula fluminea hydrolyzates obtained by using different proteases was evaluated by measuring the scavenging activity on free radical DPPH and by oxygen radical absorbance capacity ex- periments.Then，the effect of the ethanol concentrations on LO2 cells as well as the protective effect of the hydroly- zates on the cytotoxicity of LO2 cells induced by ethanol was assessed by using the methyl thiazolyl tetrazolium (MTT) assay and by determining the activities of alanine transaminase and aspartate transaminase.Finally,the a- mino acid composition of the sample,which is the most potent antioxidant,was analyzed.The results show that the hydrolyzates have certain antioxidant ability,and that,the hydrolyzates of the concentration of 300 ～400mg/L have no cytotoxic effect on the proliferation of LO2 cells,and they can increase the cell viability of the ethanol-induced LO2 cells,of which the protective effects of the pancreatin and flavourzyme hydrolyzates are significantly better than the other ones.Meanwhile,the pancreatin and flavourzyme hydrolyzates can significantly inhibit the ethanol-in- duced ALT and AST release in LO2 cells,and the contents of the antioxidant amino acids in the pancreatin and fla- vourzyme hydrolyzates are respectively 75. 69% and 75. 06%.