华南理工大学学报(自然科学版) ›› 2003, Vol. 31 ›› Issue (11): 30-32,36.

• • 上一篇    下一篇

低频超声对豆粕蛋白浸出率及SPI 功能特性的影响

杨晓泉 熊 犍 陈 中 朱建华   

  1. 华南理工大学 食品与生物工程学院‚广东 广州510640
  • 出版日期:2003-11-20 发布日期:2022-06-09
  • 作者简介:杨晓泉(1965-)‚男‚博士‚副教授‚主要从事 食品生物化学研究.
  • 基金资助:
    广东省“十五”攻关项目(A20301);国家“十五” 攻关项目(2001BA501A02)

Effects of Ultrasonification on Protein Extraction of Defatted Soy Flakes and Functionality of SPI

Y ang Xiao-quan Xiong Jian Chen Zhong Zhu Jian-hua   

  1. College of Food &Bioengineering‚South China Univ.of Tech.‚Guangzhou510640‚China
  • Online:2003-11-20 Published:2022-06-09

摘要: 研究低频超声对脱脂豆粕蛋白浸出率及大豆分离蛋白(SPI)功能特性的影响.实 验结果表明:低频超声可有效提高脱脂豆粕的蛋白浸出率‚用300W、12 Hz 低频超声处 理8min‚高温和低温脱脂豆粕的蛋白浸出率分别为73.43%和90.28%‚而45℃加热搅 拌提取1h‚高温和低温脱脂豆粕的蛋白质浸出率仅为49.58%和66.54%;SDS-PAGE 和 GPC 分析显示‚低频超声主要影响大豆的7S 蛋白组分‚并使其发生聚合;低频超声还 能明显改善 SPI 的功能特性‚SPI 溶液经过低频超声处理‚乳化活性增加‚但粘度下降.

关键词: 超声波, 大豆蛋白, 浸出率, 功能特性

Abstract: The ultrasonic extraction of soy protein from defatted soy flakes and the effects of ultrasonic treatment on the functionality of soy protein isolates (SPI) were studied.The results show that the ultrasonic treatment could obviously increase the extraction of protein from different denatured defatted soy flakes.The protein extraction of defatted soy flakes and white flakes with ultrasonic treatment at300● W and12●kHz for8●min were73.43% and90.28% respectively‚but only49.58% and66.54% with conventional stirring extraction at 45 ℃ for 1 h.SDS-PAGE and GPC analyses show that ultrasonic treatment mainly affects the 7S components of soy protein.The ultrasonic treatment can also improve the functionality of soy protein isolates (SPI) obviously.The emulsifying property of SPI increased while the viscosity decreased with ultrasonic treatment.

Key words: ultrasonification, soy protein, extraction, functionality

中图分类号: