关键词: 腺病毒载体, 核心结合因子, 柯氏质粒, 同源重组

Abstract:

In order to investigate the efect of hCbfal adenovirus vector on the artificial bone transplant. hCbfal cDNA gene was cloned to construct the recombinant adenovirus vector. In the construction. the target fragment of hCbhl cDNA was first inserted into cosmid PaxCAwt and then cotransferred into 293 cells with adenovirus gene DNA TPC(Terminal Protein Complex)by means of homologous recombination to construct the recombinant adeno-viru s vector.The positive clones of the recombinants were screened by enzyme digest identifi cation and were then treated by sequencing and purifying.A concentration of viral liquids of 2 x 10⁹ PFU/mL was thus detected,which led to the successful construction of hCbhl recombinant adenovirus vector. The present study lays a foandation for the further apphcation of the vector to the transgenic therapy for artificial bone transplant

Key words: adenovirus vector, Cbfal, cosmid, homologous recombination