华南理工大学学报(自然科学版) ›› 2015, Vol. 43 ›› Issue (6): 142-146.doi: 10.3969/j.issn.1000-565X.2015.06.022

• 生物工程 • 上一篇    

复合酶漱口液消减牙菌斑的效果分析

陈谷 雷海金 王宏 陈聪聪 向佳佳 张少波   

  1. 华南理工大学 轻工与食品学院,广东 广州 510640
  • 收稿日期:2014-07-03 修回日期:2014-11-21 出版日期:2015-06-25 发布日期:2015-05-04
  • 通信作者: 陈谷(1973-),女,博士,副教授,主要从事分子生物学与微生物生理生化研究. E-mail:chengu@scut.edu.cn
  • 作者简介:陈谷(1973-),女,博士,副教授,主要从事分子生物学与微生物生理生化研究.
  • 基金资助:
    国家自然科学基金资助项目(31270085)

Analysis of Elimination Effect of Enzyme-Combined Mouthwash on Dental Plague

Chen Gu Lei Hai-jin Wang Hong Chen Cong-cong Xiang Jia-jia Zhang Shao-bo   

  1. School of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,Guangdong,China
  • Received:2014-07-03 Revised:2014-11-21 Online:2015-06-25 Published:2015-05-04
  • Contact: 陈谷(1973-),女,博士,副教授,主要从事分子生物学与微生物生理生化研究. E-mail:chengu@scut.edu.cn
  • About author:陈谷(1973-),女,博士,副教授,主要从事分子生物学与微生物生理生化研究.
  • Supported by:
    Supported by the National Natural Science Foundation of China (31270085)

摘要: 龋齿的发生与牙齿表面附着的牙菌斑和细菌的作用密切相关,文中旨在通过复合酶液降解葡聚糖和淀粉类物质来消除牙菌斑的存在基础,考察去除牙菌斑的效果. 实验通过测定 20 名志愿者使用含不同浓度的复合酶漱口液、不含酶漱口液(无酶对照组)及市售精油类漱口液前后的 Tureskey 改良的 Q-H 菌斑指数的改变情况,考察 β-葡聚糖酶和α-淀粉酶复合酶漱口液降解牙菌斑的效果. 统计学结果显示含酶量大于 0.5 mg/L 时,牙菌斑降低率显著优于无酶对照组;且含酶量大于 5.0 mg/L 时,牙菌斑降低率优于某市售精油类漱口液. 结果表明含酶漱口液对抑制牙菌斑的作用效果明显. 文中研究为龋齿的日常防治提供了理论依据.

关键词: 牙菌斑, Q-H 菌斑指数, 复合酶漱口液, β-葡聚糖酶, α-淀粉酶

Abstract: Dental caries is closely related to dental plaque,a biofilm in which bacteria attach to the teeth surface.This paper deals with the feasibility of eliminating dental plague by using a kind of mouthwash in which combined enzymes are used to degrade glucan and starch. In the investigation,the Tureskey modified Q-H indexes of 20 vo-lunteers were measured to compare the elimination effect of the enzyme-combined mouthwash,the mouthwash with-out enzymes and the commercially available mouthwash with essential oil. Statistical results indicate that,when the enzyme content is higher than 0. 5mg/L,the mouthwash containing β-glucanase and α-amylase is more effec-tive than the one without enzymes; and that,when the enzyme content is higher than 5. 0mg/L,the enzyme-com-bined mouthwash presents a dental plague elimination rate higher than that of the commercially available mouth-wash. It is thus concluded that the mouthwash with combined enzymes is effective in eliminating dental plague.This research lays a theoretical foundation for developing products for the daily prevention of dental caries.

Key words: dental plague, Q-H dental plague index, enzyme-combined mouthwash, β-glucanase, α-amylase