广东凉茶作用于大鼠的血清代谢组学研究

华南理工大学学报(自然科学版) ›› 2012, Vol. 40 ›› Issue (12): 145-150.

广东凉茶作用于大鼠的血清代谢组学研究

尤蓉李冰徐振波李琳^†

华南理工大学轻工与食品学院，广东广州 510640

收稿日期:2012-04-19 修回日期:2012-05-23 出版日期:2012-12-25 发布日期:2012-11-02
通信作者: 李琳(1962-)，男，教授，博士生导师，主要从事糖类物质及其药物制备与生物利用研究．E-mail:felinli@ scut．edu．cn E-mail:yourong@ scnu.edu.cn
作者简介:尤蓉(1976-) ，女，在职博士生，讲师，主要从事代谢组学研究．
基金资助:
国家"973”计划项目( 2012CB720800)

Serum Metabonomics Investigation into Responses of Rats to Cantonese Herbal Tea

You Rong Li Bing Xu Zhen-bo Li Lin

School of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,Guangdong,China

Received:2012-04-19 Revised:2012-05-23 Online:2012-12-25 Published:2012-11-02
Contact: 李琳(1962-)，男，教授，博士生导师，主要从事糖类物质及其药物制备与生物利用研究．E-mail:felinli@ scut．edu．cn E-mail:yourong@ scnu.edu.cn
About author:尤蓉(1976-) ，女，在职博士生，讲师，主要从事代谢组学研究．
Supported by:
国家"973”计划项目( 2012CB720800)

摘要/Abstract

摘要： 传统中医理论认为，广东凉茶具有清热祛湿等多种功效．但由于其成分异常复杂，难以利用传统评价手段对其作用机制进行全面分析．文中引入代谢组学的研究策略，即采用稳定性和重复性俱佳的核磁共振技术( NMR) 获得大鼠血清样本中代谢物指纹图谱的“全景”数据，然后对其进行多元数据分析，识别差异标志物．结果表明，长期饮用广东凉茶使大鼠体内的脂类代谢、糖代谢及能量代谢发生变化，且其代谢响应呈现阶段性特征．本研究为深入解析广东凉茶的作用机制和建立科学的凉茶评价系统提供了实验依据．

关键词: 广东凉茶, 代谢组学, 核磁共振技术, 多元数据分析

Abstract:

As pointed out by the traditional Chinese medicine theory,Cantonese Herbal Tea ( CHT) is of various functions such as heat clearing and dampness removing. However,the corresponding action mechanism is hard to evaluate through traditional assessments due to its extremely complex components. In order to solve this problem,the NMR technology with stability and repeatability is employed to obtain the panoramic view of the fingerprints of the metabolites in the serum of rats,and a multivariate data analysis as well as an identification of discriminative biomarkers is carried out. It is found that long-term CHT intake modulates the metabolisms of fats,carbohydrates and energy in rats in a stage form. This study provides an approach to the deep analysis of action mechanism of CHT and to the construction of a scientific evaluation system of CHT.

Key words: Cantonese Herbal Tea, metabonomics, NMR technology, multivariate data analysis

尤蓉李冰徐振波李琳. 广东凉茶作用于大鼠的血清代谢组学研究[J]. 华南理工大学学报(自然科学版), 2012, 40(12): 145-150.

You Rong Li Bing Xu Zhen-bo Li Lin. Serum Metabonomics Investigation into Responses of Rats to Cantonese Herbal Tea[J]. Journal of South China University of Technology(Natural Science Edition), 2012, 40(12): 145-150.