华南理工大学学报(自然科学版) ›› 2012, Vol. 40 ›› Issue (12): 139-144.

• 生物工程 • 上一篇    下一篇

S7 木聚糖酶辅助非木浆DQP 漂白的效果

林影 张娜 郑穗平 韩双艳   

  1. 华南理工大学 生物科学与工程学院,广东 广州 510006
  • 收稿日期:2012-05-18 修回日期:2012-07-25 出版日期:2012-12-25 发布日期:2012-11-02
  • 通信作者: 韩双艳(1976-),女,副教授,硕士生导师,主要从事酶工程研究.E-mail:syhan@scut.edu.cn E-mail:feylin@ scut.edu.cn
  • 作者简介:林影(1962-) ,女,教授,博士生导师,主要从事微生物酶学研究.
  • 基金资助:

    广东省工业攻关项目( 2010A010500003)

Performance of Xylanase S7 for DQP Bleaching of Non-Wood Kraft Pulps

Lin Ying  Zhang Na  Zheng Sui-ping  Han Shuang-yan   

  1. School of Biological Science and Engineering,South China University of Technology,Guangzhou 510006,Guangdong,China
  • Received:2012-05-18 Revised:2012-07-25 Online:2012-12-25 Published:2012-11-02
  • Contact: 韩双艳(1976-),女,副教授,硕士生导师,主要从事酶工程研究.E-mail:syhan@scut.edu.cn E-mail:feylin@ scut.edu.cn
  • About author:林影(1962-) ,女,教授,博士生导师,主要从事微生物酶学研究.
  • Supported by:

    广东省工业攻关项目( 2010A010500003)

摘要: 使用重组毕赤酵母基因工程菌发酵所得的S7 木聚糖酶对麦草浆、芦苇浆、竹浆3 种非木浆进行预处理( 70℃,pH9.0) ,随后进行无元素氯DQP 漂白,并与两种商品酶AU-PE89和Pulpzyme HC 的助漂效果进行比较.结果表明,使用等量的化学助漂剂时,在高温强碱的条件下进行预处理的S7 木聚糖酶,可将非木浆白度提高1%ISO 以上,并可降低非木浆的卡伯值,提高非木浆的物理强度,效果与商品酶相近.通过扫描电子显微镜观察发现,经酶处理的纤维表面出现明显变化.

关键词: 木聚糖酶, 非木浆, DQP 漂白, 生物漂白

Abstract:

Three kinds of non-wood pulps,namely,wheat straw pulp,reed pulp and bamboo pulp,were pretreated at 70℃ and pH9.0 with xylanase S7 secreted by recombinant Pichia pastoris and were then treated with chlorinefree DQP bleaching. Then,the aid-bleaching effect of xylanase S7 was compared with those of two kinds of commercial xylanases AU-PE89 and Pulpzyme HC. The results indicate that,with the same bleaching reagent consumption,the non-wood pulps treated with xylanase S7 are of a brightness increment of more than 1%ISO,a lower Kappa number and better physical properties,which means that xylanase S7 possesses an aid-bleaching effect similar to those of the commercial enzymes. Moreover,it is found from SEM results that,after the enzymatic pre-treatment,the fiber surface changes obviously.

Key words: xylanase, non-wood pulp, DQP bleaching, bio-bleaching

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