Abstract:

Artificial morphine antigen was chemically synthesized and was then used to immunize the New Zealand rabbits to prepare the polyclinal anti -morphine antibody. Mterwards , the morphine antigen is respectively coupled with two protein carriers( BSA and OV A) by means of mixed acid anhydride method. It is proved by UV scan and protein electrophoresis that the artificial morphine antigen is successfully synthesized. Moreover, it is proved by EUSA results that the rabbit serum with high dilute degree is prepared , and that there is a high-specificity reaction between the polyclonal antibody and the antigen. Both the antigen and the antibody can be applied to the research on morphine immunoassay.