关键词: 酶改性, 酶分子修饰, 酶固定化, 酶非水相催化, 酶定向进化

Abstract:

Although enzymes possess high catalytic efficiency and good substrate specificity , their activities and stabilities often can not meet the requirements of application. In order to improve the catalytic properties of enzymes , the author and his group have devoted themselves to the researches of enzymatic molecular modification , enzyme immobilization , non-aqueous enzymatic catalysis and directed enzyme evolution in the past two decades , finding that the catalytic properties of enzymes such as activity and stability can significantly be improved by enzyme improving technology. This paper reviews the achievement and progress in the field of enzyme improving technology made by the author and his group.

Key words: enzyme improving technology, enzymatic molecular modification, enzyme immobilization, non-aqueous enzymatic catalysis, directed enzyme evolution