华南理工大学学报(自然科学版) ›› 2006, Vol. 34 ›› Issue (5): 68-71.

• 生物工程 • 上一篇    下一篇

超声作用下的酶促废油脂转酯反应

吴虹 宗敏华 娄文勇 陈志锋   

  1. 华南理工大学 生物工程系,广东 广州 510640
  • 收稿日期:2005-03-28 出版日期:2006-05-25 发布日期:2006-05-25
  • 通信作者: 吴虹(1971-),女,讲师,主要从事生物合成与生物转化的研究 E-mail:bbhwn@scut.edu.cn
  • 作者简介:吴虹(1971-),女,讲师,主要从事生物合成与生物转化的研究
  • 基金资助:

    广东省科技计划项目(2003C33102);广州市科技计划项目(2005Z3-130471)

Enzymatic Transesteri6catiOn of Waste Oil Under Ultrasonic Irradiation

Wu Hong  zong Min-hua  Lou Wen-yong Chen Zhi-fen   

  1. Dept.of Biotechnology,South China UniV of Tech.,Guangzhou 510640,Guangdong,China
  • Received:2005-03-28 Online:2006-05-25 Published:2006-05-25
  • Contact: 吴虹(1971-),女,讲师,主要从事生物合成与生物转化的研究 E-mail:bbhwn@scut.edu.cn
  • About author:吴虹(1971-),女,讲师,主要从事生物合成与生物转化的研究
  • Supported by:

    广东省科技计划项目(2003C33102);广州市科技计划项目(2005Z3-130471)

摘要: 探讨了超声对无溶剂体系中固定化脂肪酶Novozym435催化废油脂转酯反应的促进作用,研究了超声功率、超声处理方式等对酶反应速度及酶操作稳定性的影响.结果表明:对酶进行适宜的超声预处理(20kHz,80 W,30min)能显著提高Novozym 435催化废油脂转酯反应的速度;经超声预处理的Novozym 435有良好的操作稳定性,反应10批次后.酶活下降甚微.

关键词: 超声辐照, 脂肪酶, 废油脂, 转酯反应

Abstract:

Aiming at the Novozym 435-catalyzed transesteri cation of waste oil in a solvent-free system under ultra.sonic irradiation.the effects of ultrasonic power and radiation mode on the reaction rate and the operation stability were investigated.It is found that the reaction rate of Novozym 435.catalyzed transesterification of waste oiL is remarkably accelerated by pretreatment of enzyme with uhrasonic irradiation at 20 kHz.80 W for 30 min.and that Novozym 435 pretreated with uhrasonic irradiation is of good operation stability because its activity weakens little even after 10 batch operations.

Key words: uhrasonic iradiation;, lipase, waste oil, transestefification