根癌农杆菌介导的玫瑰茄细胞遗传转化

华南理工大学学报（自然科学版） ›› 2006, Vol. 34 ›› Issue (5): 43-47,52.

根癌农杆菌介导的玫瑰茄细胞遗传转化

谢秀祯林俏慧郭成栓郭勇^†

华南理工大学生物科学与工程学院，广东广州 510640

收稿日期:2005-07-12 出版日期:2006-05-25 发布日期:2006-05-25
通信作者: 郭勇，教授，E—mail：btyguo@ scut．edu．cn E-mail:xiexiuzhen@sina.com
作者简介:谢秀祯(1960-)，男，在职博士生，海南师范大学教授，主要从事基因工程的研究
基金资助:
广东省重点科技攻关项目(A301020201)

Agrobacterium tumefaciens-Mediated Genetic Transformation of Roselle Cell

Xie Xiu-zhen Lin Qiao-hui Guo Cheng-shuan Guo Yong

College of Biological Science and Engineering,South China Univ.of Tech.,Guangzhou 510640,Guangdong,China

Received:2005-07-12 Online:2006-05-25 Published:2006-05-25
Contact: 郭勇，教授，E—mail：btyguo@ scut．edu．cn E-mail:xiexiuzhen@sina.com
About author:谢秀祯(1960-)，男，在职博士生，海南师范大学教授，主要从事基因工程的研究
Supported by:
广东省重点科技攻关项目(A301020201)

摘要/Abstract

摘要： 以玫瑰茄无茵苗的子叶和下胚轴为受体材料，农杆茵LBA4404和EHA105为供体菌株，对玫瑰茄细胞外植体遗传转化的基本条件进行研究，建立了一个高效的玫瑰茄细胞遗传转化体系．利用这一转化体系获得了2个稳定表达新霉素磷酸转移酶活性的玫瑰茄转化细胞系．β-葡萄糖苷酸酶活性的组织化学检测和PCR扩增鉴定的结果表明，外植体总转化率达29.4%．试验结果还显示农杆茵LBA4404菌株较适合玫瑰茄外植体的转化，转化时不需添加乙酰丁香酮．

关键词: 玫瑰茄, 根癌农杆菌, 遗传转化, β-葡萄糖苷酸酶, 新霉素磷酸转移酶

Abstract:

By using Agrobacterium tumefaciens strains LBA4404 and EHA 105 as the donor strains.the cotyledon and hypocotyl of sterile seedling of roselle as the recipient materials.the basic conditions for the genetic transforma.tion of Roselle cell in vitro were investigated.Then a high-efficiency genetic transformation system was established ,by which two transgenic Roselle cell lines that stably express the activity of neomycin phosphotI_ans rase Ⅱ were ob tained.The results of β-glucuronidase histochemical assay and PC R amplification demonstrate that the overall trans-form ation rate of the explant reaches 29.4% ,and that,as compared with the strain of EHA105.the strain of LBA4404 is more suitable for the transform ation of Roselle explants because no acetosyringone is needed

Key words: Roselle, Agrobacterium tumCaciens, genetic transformation, β-glucuronidase, neomycin phosphotrans feraseⅡ

谢秀祯林俏慧郭成栓郭勇. 根癌农杆菌介导的玫瑰茄细胞遗传转化[J]. 华南理工大学学报（自然科学版）, 2006, 34(5): 43-47,52.

Xie Xiu-zhen Lin Qiao-hui Guo Cheng-shuan Guo Yong. Agrobacterium tumefaciens-Mediated Genetic Transformation of Roselle Cell[J]. Journal of South China University of Technology (Natural Science Edition), 2006, 34(5): 43-47,52.

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