Determination of the Antibiotic Susceptibilities of
Four Marine Pathogenic Vibrios

华南理工大学学报(自然科学版) ›› 2003, Vol. 31 ›› Issue (9): 70-75.

Determination of the Antibiotic Susceptibilities of Four Marine Pathogenic Vibrios

王启军1 张水华1 成昌根2 徐宝军2 李长田2 白承泳3

1．华南理工大学食品与生物工程学院广东广州510640； 2．忠南大学校农业生命科学大学食品工学科韩国大田305－764； 3．大德生物技术有限公司韩国大田305－764

出版日期:2003-09-20 发布日期:2022-05-23
作者简介:王启军（1972－）男博士讲师主要从事生物工程与食品分析的研究．

Determination of the Antibiotic Susceptibilities of Four Marine Pathogenic Vibrios

Wang Q-i jun 1 Zhang Shu-i hua 1 Sung Chang-keun 2 Xu Bao-jun 2 Li Chang-tian 2 Baek Seoung-young 3

1．College of Food ＆Biological EngineeringSouth China Univ．of Tech．Guangzhou510640China； 2．Dept．of Food Sci．＆Tech．College of Agricutural Life Sci．Chungnan National Univ．Taejon305－764Korea； 3．Daedok Biotechnique Inc．Taejon305－764Korea

Online:2003-09-20 Published:2022-05-23
About author:Wang Q-i jun （born in1972）malePh．Dlecturermainly researches on bioengineering and food ana-lysis．

摘要/Abstract

摘要： 通过实验筛选出了适于特异性的同步检测酸式和内酯式莫奈呵啉 K 的薄层色谱法（TLC）．最佳薄层色谱条件为：薄层板采用德国 Merck 公司的化学修饰薄层板 Silica Gel60RP－18F2540．5mm；展开剂系统采用 V（氯仿）∶V（甲醇）∶V（氨水）＝25∶3∶1；炭化试剂采用30％（体积分数）的硫酸乙醇溶液；紫外检测波长为365nm．该法实现了酸式和内脂式莫奈呵啉 K 的检测特异性与同步性并将检测灵敏度提高到绝对检测量30ng．该法适用于任何含莫奈呵啉 K 的样品的检测特别适用于大批量经常性的定性或半定量莫奈呵啉 K 的检测．

关键词: 薄层色谱, 内酯式莫奈呵啉 K, 酸式莫奈呵啉 K

Abstract: A novel and sensitive thin-layer chromatography（TLC） method for specific and simultaneous detection of both lactone form Monacolin K and acid form Monacolin K is developed in this study．The TLC works in the following conditions： The TLC plate was Silica Gel60RP－18F2540．5mmchemically modified layerMerck Germany；The solvent system was chloroform∶methanol ∶ammonium hydroxide＝25∶3∶1（volume ratio）；Monitoring the TLC under365nm after heating it with a slight coating of the charring reagent 30％（volume fraction） H2SO4ethanol on the TLC plateallowed the specific detection of Monacolin K and the low detection limit was first time brought down to30ng．The present TLC method is suitable for the routine qualitative and sem-i quantitative analysis of the bulk materials containing Monacolin K．

Key words: thin-layer chromatograhpy, lactone form Monacolin K, acid form Monacolin K

中图分类号:

R151．3

王启军张水华成昌根徐宝军李长田白承泳. Determination of the Antibiotic Susceptibilities of Four Marine Pathogenic Vibrios[J]. 华南理工大学学报(自然科学版), 2003, 31(9): 70-75.

Wang Q-i jun Zhang Shu-i hua Sung Chang-keun Xu Bao-jun Li Chang-tian Baek Seoung-young . Determination of the Antibiotic Susceptibilities of Four Marine Pathogenic Vibrios[J]. Journal of South China University of Technology(Natural Science Edition), 2003, 31(9): 70-75.

[1]	郑必胜, 杨文涵, 徐秋雄, 等. 阿魏酸及其结肠代谢物的抗肿瘤活性[J]. 华南理工大学学报(自然科学版), 2022, 50(8): 30-40.
[2]	苏健裕胡菡吴萍孟晓风徐振波郑华德. 杨梅酮的体外抗氧化及抗肿瘤活性[J]. 华南理工大学学报（自然科学版）, 2019, 47(3): 101-108,152.

Determination of the Antibiotic Susceptibilities of Four Marine Pathogenic Vibrios

Determination of the Antibiotic Susceptibilities of Four Marine Pathogenic Vibrios

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价