关键词: 骨组织工程, 人骨髓间充质干细胞, 聚乳酸－羟基乙酸, 磷酸三钙, 细胞增殖, 成骨分化, 生物相容性

Abstract:

In order to verify the directional osteogenic differentiation of human mesenchymal stem cells (hMSCs),the corresponding experiments were conducted with the hMSCs cultured in the osteogenic differentiation medium as the osteogenic differentiation group and those in the complete medium as a control group.Von Kassa experiment shows that hMSCs has a good osteogenic differentiation ability with obvious calcium nodules.Alkaline phosphatase (ALP) test demonstrates that the ALP activity of the osteogenic differentiation group is higher than that of the con- trol group (P ＜0.01),and reaches a peak in twelve days and then enters into a plateau phase.In order to verify the biocompatibility of hMSCs with poly lactide- co- glycolide (PLGA) /TCP,the corresponding analyses are made with the hMSCs cultured in PLGA/TCP as the 3D culture group and those on plate as the 2D control group.SEM results show that adhesion,proliferation and osteogenic differentiation of hMSCs occur in PLGA/TCP.MTT test in- dicates that the cell proliferation rate of the 3D culture group in five and seven days (P ＜0.01) is obviously higher than those of the 2D control group.ALP test also demonstrates that the osteogenic differentiation of the 3D culture group in three,five and seven days (P ＜0.05) are higher than those of the 2D control group,which indicates that PLGA/TCP possesses good cellular biocompatibility with hMSCs and they have a potential to produce tissue engi- neering bones.

Key words: bone tissue engineering, human mesenchymal stem cells, poly lactide- co- glycolide, tricalcium phos- phate, cell proliferation, osteogenic differentiation, biocompatibility