华南理工大学学报(自然科学版) ›› 2014, Vol. 42 ›› Issue (6): 129-135.doi: 10.3969/j.issn.1000-565X.2014.06.020

• 食品科学与技术 • 上一篇    下一篇

干燥方式对脱酰胺小麦面筋蛋白特性的影响

仇超颖 孙为正 崔春 赵谋明   

  1. 华南理工大学 轻工与食品学院,广东 广州 510640
  • 收稿日期:2013-08-19 修回日期:2014-02-15 出版日期:2014-06-25 发布日期:2014-05-01
  • 通信作者: 赵谋明(1964-),男,教授,博士生导师,主要从事食品生物技术、蛋白质化学与工程等的研究. E-mail:femmzhao@scut.edu.cn
  • 作者简介:仇超颖(1988-),女,博士生,主要从事蛋白质改性及应用研究.E-mail:qiucy882@gmail.com
  • 基金资助:

    国家 “863” 计划项目(2013AA102201);国家 “十二五” 科技支撑计划项目(2012BAD37B08)

Effects of Drying Methods on Characteristics of Deamidated Wheat Gluten

Qiu Chao- ying Sun Wei- zheng Cui Chun Zhao Mou- ming   

  1. School of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,Guangdong,China
  • Received:2013-08-19 Revised:2014-02-15 Online:2014-06-25 Published:2014-05-01
  • Contact: 赵谋明(1964-),男,教授,博士生导师,主要从事食品生物技术、蛋白质化学与工程等的研究. E-mail:femmzhao@scut.edu.cn
  • About author:仇超颖(1988-),女,博士生,主要从事蛋白质改性及应用研究.E-mail:qiucy882@gmail.com
  • Supported by:

    国家 “863” 计划项目(2013AA102201);国家 “十二五” 科技支撑计划项目(2012BAD37B08)

摘要: 采用喷雾干燥和冷冻干燥两种方法制得了脱酰胺小麦面筋蛋白,通过红外光谱分析、拉曼光谱分析及轴对称型分析法表征了所制得的面筋蛋白的结构及界面性质的变化,并对其乳化和起泡性进行了分析.结果表明:冷冻干燥法能更好地保持蛋白形态;脱酰胺后蛋白基团强度发生变化;冷冻干燥法所得蛋白的结构展开程度较高;喷雾干燥法所得蛋白的起泡性及乳化性均高于冷冻干燥法所得蛋白,但起泡稳定性及乳化稳定性降低;脱酰胺面筋蛋白能较好地降低界面张力,其中喷雾干燥法所得蛋白降低界面张力的能力更高,但界面膜弹性低于冷冻干燥法所得蛋白,可能是由于喷雾干燥中形成了不利于界面膜稳定性的不溶性组分.

关键词: 脱酰胺小麦面筋蛋白, 喷雾干燥, 冷冻干燥, 结构, 起泡性, 乳化性, 界面特性

Abstract:

Deamidated wheat gluten was prepared via spray drying and freeze drying,and the structure as well asthe interfacial properties of the product was characterized by means of FT- IR,Raman spectroscopy and axisymmet-ric drop shape analysis.Then,the emulsifying and foaming properties of the gluten were analyzed.The results showthat (1) freeze drying helps to maintain the protein structure well; (2) the molecular vibration intensity changesafter deamidation; (3) freeze drying may result in greater structure unfolding of protein; (4) the samples obtainedby spray drying possess higher foaming and emulsifying ability but lower stability; and (5) deamidation helps toreduce the surface intension of gluten,and the samples obtained by spray drying are more effective in reducing thesurface tension but is of lower interfacial film elasticity,as compared with the samples obtained by freeze drying,which might be caused by the formation of insoluble protein that lowers the stability of the interfacial film.

Key words: deamidated wheat gluten, spray drying, freeze drying, structure, foaming property, emulsifying prop-erty, interfacial characteristic

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