软骨及姜黄联合葛根薏仁提取物对膝骨关节炎大鼠软骨损伤的改善作用

doi:10.12141/j.issn.1000-565X.220815

华南理工大学学报(自然科学版) ›› 2024, Vol. 52 ›› Issue (4): 17-25.doi: 10.12141/j.issn.1000-565X.220815

软骨及姜黄联合葛根薏仁提取物对膝骨关节炎大鼠软骨损伤的改善作用

丁刘刚¹^,² 关婷³ 缪金典³ 方兰婷³ 侯少贞³ 苏国万^1† 周勇²

^1.华南理工大学食品科学与工程学院, 广东广州 510640
^2.无限极(中国)有限公司, 广东广州 510665
^3.广州中医药大学中药学院, 广东广州 510006

收稿日期:2022-12-15 出版日期:2024-04-25 发布日期:2023-10-27
通信作者: 苏国万（1981-），男，博士，教授级高级工程师，主要从事食品生物技术研究 E-mail:fegwsu@scut.edu.cn
作者简介:丁刘刚（1982-），男，博士生，主要从事食品生物技术研究。E-mail:109277322@qq.com
基金资助:
广东省重点领域研发计划项目(2020B020226005);华南理工大学中央高校基本科研业务费专项资金资助项目(2022ZYGXZR0074)

Improvement Effects of Co-administration of Cartilage Extract, Turmeric Extract, Pueraria Lobata and Coix Seed Extract on Cartilage Damage of Rats with Knee Osteoarthritis

DING Liugang¹^,² GUAN Ting³ MIAO Jindian³ FANG Lanting³ HOU Shaozhen³ SU Guowan¹ ZHOU Yong²

^1.School of Food Science and Engineering，South China University of Technology，Guangzhou 510640，Guangdong，China
^2.Infinitus （China） Company Ltd. ，Guangzhou 510665，Guangdong，China
^3.School of Pharmaceutical Science，Guangzhou University of Chinese Medicine，Guangzhou 510006，Guangdong，China

Received:2022-12-15 Online:2024-04-25 Published:2023-10-27
Contact: 苏国万（1981-），男，博士，教授级高级工程师，主要从事食品生物技术研究 E-mail:fegwsu@scut.edu.cn
About author:丁刘刚（1982-），男，博士生，主要从事食品生物技术研究。E-mail:109277322@qq.com
Supported by:
the Key Field R&D Program of Guangdong Province(2020B020226005)

摘要/Abstract

摘要：

系统研究了姜黄提取物、软骨提取物、葛根薏仁提取物单独及联合使用对膝骨关节炎大鼠软骨损伤的改善作用，主要从减少膝关节肿胀（炎症）、促进软骨细胞修复和关节基质修复方面，研究了3种提取物在大鼠膝骨关节炎中的协同效应。结果显示：姜黄提取物在抑制关节肿胀、改善软骨损伤方面有较明显的作用，具体表现在能较好地改善Ⅱ型胶原蛋白（Collagen Ⅱ）、基质金属蛋白酶3（MMP3）、软骨寡聚基质蛋白（COMP）、肿瘤坏死因子-α（TNF-α）及超氧化物歧化酶（SOD）的水平；软骨提取物主要通过改善MMP3、COMP、TNF-α、前列腺素E2（PGE2）及SOD水平起到明显改善关节炎病变的效果；葛根薏仁提取物能够提高Collagen Ⅱ及SOD水平，抑制MMP3、COMP、TNF-α和白细胞介素1β（IL-1β）的升高，从而改善大鼠膝关节病变程度；3种提取物的复合使用对大鼠膝关节软骨的修复作用最为突出，且对各生化指标的异常改变均有更佳的改善效果。由此可见，通过对姜黄提取物、软骨提取物及葛根薏仁提取物的比例优化，可开发出具有显著抗炎和修复软骨作用的功能性食品，为实现“健康中国2030”添砖加瓦。

关键词: 软骨提取物, 姜黄提取物, 葛根薏仁提取物, 膝骨关节炎, 软骨修复

Abstract:

This paper studied the synergistic effects of turmeric extract, cartilage extract, Pueraria lobata and Coix (Coix lacryma-jobi) seed extract alone and the combination on the improvement of cartilage damage of rats with knee osteoarthritis. The synergistic effects of the three extracts in rat knee osteoarthritis were evaluated mainly by reducing knee swelling (inflammation), protecting chondrocyte and repairing joint cartilage. The results show that the turmeric extract has a more obvious effect in inhibiting joint swelling and improving cartilage damage, which is specifically manifested in the ability to better improve the levels of Collagen Ⅱ, matrix metalloproteinase 3 (MMP3), cartilage oligomeric matrix protein (COMP), tumor necrosis factor-alpha (TNF-α) and superoxide dismutase (SOD). The cartilage extract significantly improves the arthritic lesions by improving the levels of MMP3, COMP, TNF-α, prostaglandin E2 (PGE2) and SOD. Pueraria lobata and Coix seed extract improves the levels of Collagen Ⅱ and SOD, inhibits the elevation of MMP3, COMP, TNF-α and interleukin 1β (IL-1β), and improves cartilage damage of rats with knee osteoarthritis. The combined use of the three extracts exhibits better effects on the repair of rat knee cartilage. These data imply that by optimizing the ratio of turmeric extract, cartilage extract and Pueraria lobata and Coix seed extract, functional foods can be developed with significant anti-inflammatory and cartilage repairing effects and contribute to the realization of “Healthy China 2030”.

Key words: cartilage extract, turmeric exact, Pueraria lobata and Coix seed extract, knee osteoarthritis, cartilage repair

中图分类号:

R684.3

丁刘刚, 关婷, 缪金典, 等. 软骨及姜黄联合葛根薏仁提取物对膝骨关节炎大鼠软骨损伤的改善作用[J]. 华南理工大学学报(自然科学版), 2024, 52(4): 17-25.

DING Liugang, GUAN Ting, MIAO Jindian, et al. Improvement Effects of Co-administration of Cartilage Extract, Turmeric Extract, Pueraria Lobata and Coix Seed Extract on Cartilage Damage of Rats with Knee Osteoarthritis[J]. Journal of South China University of Technology(Natural Science Edition), 2024, 52(4): 17-25.

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参考文献 21

1	ALTAY M A， ERTÜRK C， BILGE A，et al ．Evaluation of prolidase activity and oxidative status in patients with knee osteoarthritis：relationships with radiographic severity and clinical parameters［J］．Rheumatology International，2015，35（10）：1725-1731.
2	GOLDRING M B， OTERO M ．Inflammation in osteoarthritis［J］．Current Opinion in Rheumatology，2011，23（5）：471-478.
3	VILÁ S ．Inflammation in osteoarthritis［J］．Puerto Rico Health Sciences Journal，2017，36（3）：123-129.
4	HÉRAUD F， HÉRAUD A， HARMAND M F ．Apoptosis in normal and osteoarthritic human articular cartilage［J］．Annals of the Rheumatic Diseases，2000，59（12）：959-965.
5	BISHNOI M， JAIN A， HURKAT P，et al ．Chondroitin sulphate：a focus on osteoarthritis［J］．Glycoconjugate Journal，2016，33（5）：693-705.
6	沈先月，李阳，张卓．姜黄素治疗骨关节炎的研究进展［J］．中华外科杂志，2021，59（6）：554-557.
	SHEN Xianyue， LI Yang， ZHANG Zhuo ．Research progress of curcumin in the treatment of osteoarthritis［J］．Chinese Journal of Surgery，2021，59（6）：554-557.
7	宋亚丽，公慧萍．姜黄素治疗骨关节炎的作用机制研究进展［J］．长治医学院学报，2021，35（6）：463-466.
	SONG Yali， GONG Huiping ．Progress on the mechanism of action of curcumin in the treatment of osteoarthritis［J］．Journal of Changzhi Medical College，2021，35（6）：463-466.
8	NAKAGAWA Y， MUKAI S， YAMADA S，et al ．The efficacy and safety of highly-bioavailable curcumin for treating knee osteoarthritis：a 6-month open-labeled prospective study［J］．Clinical Medicine Insights：Arthritis and Musculoskeletal Disorders，2020，13：1-8.
9	张春雷，李冀．膝骨关节炎的中医药治疗进展［J］．中医药学报，2022，50（1）：106-110.
	ZHANG Chunlei， LI Ji ．Progress in the treatment of osteoarthritis of the knee with traditional Chinese medicine［J］．Acta Chinese Medicine and Pharmacology，2022，50（1）：106-110.
10	HUANG X， PEI W， NI B，et al ．Chondroprotective and antiarthritic effects of galangin in osteoarthritis：an in vitro and in vivo study［J］．European Journal of Pharmacology，2021，906：174232/1-7.
11	SOHEL M J， TADASHI Y， MAKOTO S，et al ．Inhibition of interleukin-1β-stimulated production of matrix metalloproteinases by hyaluronan via CD44 in human articular cartilage［J］．Arthritis & Rheumatism，2004，50（2）：516-525.
12	BARANOVSKY D S， LYUNDUP A V， BALYASIN M V，et al ．Interleukin IL-1β stimulates cartilage scaffold revitalization in vitro with human nasal chondrocytes［J］．Russian Journal of Transplantology and Artificial Organs，2019，21（4）：88-95.
13	KOBAYASHI M， SQUIRES G R， MOUSA A，et al ．Role of interleukin-1 and tumor necrosis factor α in matrix degradation of human osteoarthritic cartilage［J］．Arthritis & Rheumatism，2005，52（1）：128-135.
14	苑文杰，宋锡国，徐翀，等．葛根素促进骨关节炎软骨细胞增殖和减轻氧化应激与炎性反应的机制研究［J］．母婴世界，2021（15）：77.
	YUAN Wenjie， SONG Xiguo， XU Chong ．Mechanism of Puerarin in promoting osteoarthritic chondrocyte proliferation and reducing oxidative stress and inflammatory response in osteoarthritis［J］．Super Baby，2021（15）：77.
15	温亚静．葛根素对ACLT诱导的骨关节炎模型的治疗效果观察［D］．哈尔滨：东北农业大学，2020.
16	柏琳，邹天琪，张成义．葛根总黄酮对佐剂性关节炎大鼠免疫器官指数、IL-1β及TNF-α的影响分析［J］．吉林医学，2021，42（6）：1298-1300.
	BO Lin， ZOU Tianqi， ZHANG Chengyi ．Analysis of the effects of total flavonoids of Pueraria lobata on immune organ index，IL-1β and TNF-α in rats with adjuvant arthritis［J］．Jilin Medical Journal，2021，42（6）：1298-1300.
17	章超，康建华，邹红．薏苡仁汤加味结合温针灸治疗风寒湿痹型类风湿性关节炎临床观察［J］．光明中医，2021，36（19）：3299-3301.
	ZHANG Chao， KANG Jianhua， ZOU Hong ．Clinical observation on coix seed decoction combined with warm acupuncture and moxibustion in the treatment of rheumatoid arthritis of wind-cold-dampness arthralgia type［J］．Guangming Journal of Chinese Medicine，2021，36（19）：3299-3301.
18	郭晋良，冯玛莉．中医药治疗痛风性关节炎用药经验探析［J］．光明中医，2021，36（10）：1543-1547.
	GUO Jinliang， FENG Mali ．Exploration on empirical medication of traditional Chinese medicine in the treatment of gouty arthritis［J］．Guangming Journal of Chinese Medicine，2021，36（10）：1543-1547.
19	王蕾，王宇，刘文俊，等．盐酸氨基葡萄糖联合硫酸软骨素治疗膝关节骨性关节炎的疗效观察［J］．医学理论与实践，2021，34（1）：79-81.
	WANG Lei， WANG Yu， LIU Wenjun，et al ．The efficacy of glucosamine hydrochloride combined with chondroitin sulfate in the treatment of osteoarthritis of the knee joints［J］．The Journal of Medical Theory and Practice，2021，34（1）：79-81.
20	王铜浩，田永刚，韩立强，等．姜黄素对骨质疏松鼠模型作用机制的研究［J］．医学信息，2022，35（8）：30-33.
	WANG Tong-hao， TIAN Yong-gang， HAN Li-qiang，et al ．Research on the mechanism of curcumin on the osteoporosis rat model［J］．Journal of Medical Information，2022，35（8）：30-33.
21	何晶，邱春媚，黄远英，等．氨糖软骨素钙姜黄复合物对骨密度的协同作用［J］．食品工业，2023，44（2）：131-134.
	HE Jing， QIU Chunmei， HUANG Yuanying，et al ．Synergistic effect of the compound of glucosamine chondroitin calcium and curcumin on bone mineral density［J］．The Food Industry，2023，44（2）：131-134.

软骨及姜黄联合葛根薏仁提取物对膝骨关节炎大鼠软骨损伤的改善作用

Improvement Effects of Co-administration of Cartilage Extract, Turmeric Extract, Pueraria Lobata and Coix Seed Extract on Cartilage Damage of Rats with Knee Osteoarthritis

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