以柠檬苦素收率为评价指标，比较了正交实验设计法、均匀设计法和响应曲面法对柠檬苦素闪式提取工艺条件的优化效果．采用最优的闪式提取工艺结合结晶法，从广州新会柑种子中提取分离出柠檬苦素，并通过高效液相色谱检测其纯度．结果表明: 获得的柠檬苦素纯度达95%; 3 种优化方法结果相近，其中响应曲面法得到的最佳提取条件下的柠檬苦素收率最高，达( 6. 65 ± 0. 01) mg /g，最佳提取条件为: 乙醇浓度71. 7%( 体积分数) 、液固比28. 8mL /g、闪提转速3953 r /min．

The homogenate extraction of limonin respectively by means of the orthogonal design,the uniform design and the response surface methodology ( RSM) ,with the limonin yield,as the evaluation index. Then,by combining the optimal process with the method of crystallization,limonin was extracted from the seeds of oranges ( Citrus reticulata Blanco) in Guangzhou. Moreover,its purity was analyzed through the high-performance liquid chromatography. The results show that the purity of the sample reaches 95%,that the effects of the three statistical methods on the yield are close to each other,and that,by means of RSM,the maximum limonin yield in up to( 6. 65 ± 0. 01) mg /g in the optimal conditions,namely,an ethanol concentration of 71. 7% ( volume fraction) ,a solvent-solid ratio of 28. 8mL /g and a rotating speed of 3953 r /min.