收稿日期: 2010-10-12
修回日期: 2010-12-29
网络出版日期: 2011-04-01
基金资助
广东省自然科学基金资助项目(10151001002000012);广东省科技计划项目(2010B060200009
Transformation of Polygonum cuspidatum Resveratrol Synthase Gene into Arabidopsis thaliana
Received date: 2010-10-12
Revised date: 2010-12-29
Online published: 2011-04-01
Supported by
广东省自然科学基金资助项目(10151001002000012);广东省科技计划项目(2010B060200009
柳忠玉 庄楚雄 生书晶 邵利 赵树进 . 虎杖白藜芦醇合酶基因转化拟南芥的研究[J]. 华南理工大学学报(自然科学版), 2011 , 39(5) : 138 -142 . DOI: 10.3969/j.issn.1000-565X.2011.05.024
In order to reveal the effectiveness of Polygonum cuspidatum resveratrol synthase(PcRS) gene in improving the resveratrol accumulation in foreign species,first,the plant expression vector pCAM1380-35S-PcRS was constructed and introduced into Arabidopsis thaliana by Agrobacterium tumefaciens by means of the floral dip me-thod.Next,the transformants were screened on the medium containing hygromycin.Then,the integration of PcRS gene into the transgenic plants was verified via the PCR and the Southern blotting.Moreover,independent homozygous transgenic Arabidopsis thaliana lines with genetical stability were obtained after the selection of T3 progenies,and the expression of the gene transferred into Arabidopsis thaliana was confirmed by Northern blotting.Finally,an end product,namely trans-piceid,was identified by HPLC,the content of which in 2-week-old plants being 136 μg/g(fresh weight) or 1 813 μg/g(dry weight).
Key words: Polygonum cuspidatum; resveratrol synthase gene; Arabidopsis; resveratrol; piceid
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