对选育的一株木腐真菌Psathyrella candolleana 进行液体深层发酵生产漆酶，通过硫酸铵盐析和离子交换色谱等纯化技术分离发酵酶液后，得到纯度较高的漆酶，并对其酶学性质进行研究． 结果表明: P． candolleana 在发酵罐中的漆酶产量最高可达9100 U/L; 分离纯化后得到漆酶活性组分，其比活力达到438. 0 U/mg，纯化倍数为13. 2，得率为50. 1%; 该漆酶蛋白分子质量约为61. 2 ku，米氏常数为16 μmol /L; 该漆酶催化反应最适温度和pH 值分别为45℃和5. 0，在4 ～ 10 ℃和pH 9. 0 的条件下漆酶活力稳定性较好，Mg²⁺、Mn²⁺、Zn²⁺ 等金属离子对其活力的影响较小．

Laccase from a newly isolated wood-rot fungus Psathyrella Candolleana,was produced via the submerged fermentation,and high-purity laccase was obtained by means of the ammonium sulfate precipitation and the ion-exchange chromatography. Then,the enzymatic properties of the laccase were also analyzed. The results indicate that the maximal activity of the laccase from P. candolleana in the fermentor reaches 9100U/L,that the active
component of the laccase achieved after 13. 2-fold purification is of a specific activity of 438. 0U/mg with a yield of 50. 1%,that the molecular mass and Michaelis constant of the laccase are respectively about 61. 2ku and 16μmol /L,and that the optimal pH value and temperature for the laccase activity are 5. 0 and 45℃,respectively. Moreover,it is also found that the laccase activity is stable under 4 ~10℃ at pH 9. 0 and is not sensitive to Mg^{2 +} ,Mn^{2 +} and Zn^{2 +} .